Chemometric Analysis Of Amino Acids And Minerals Composition For Traceability Of Edible Bird’s Nest
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Date
2015-08
Authors
ENG KENG, SEOW
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Abstract
Edible bird’s nest (EBN) is a high-priced tonic food favored by the Chinese community. Consumers have been deceived into buying house-farmed EBN at premium price of cave-harvested EBN. In the present study, a reliable and accurate method was proposed to differentiate EBN of house and cave origin. Calcium (Ca), sodium (Na), tyrosine (TYR) and glutamic acid (GLU) were proposed as promising discriminating variables for differentiating between house and cave EBN samples. Similar approach was applied for provenance traceability of house EBN from different regions but the clusters formed were not distinctly separated. Thus, amino acids and minerals profiles have been found not able to serve as good indicators for provenance traceability of EBN. The amino acids and minerals profile determined by gas chromatography-mass spectrometry (GC-MS) and inductively coupled plasma-optical emission spectrometry (ICP-OES), respectively were analyzed using Pearson correlation analysis, principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA). There were significant different correlation patterns seen between different amino acids pair and minerals pair within each EBN group. PCA was applied to study possible clustering, wherein house and cave EBN were separated by two principal components (PC), PC1 and PC3 which explains 43.6% and 12.6% of the total variability in data set, respectively. The model constructed by OPLS-DA was found to be a promising tool with high predictive
ability of 89.5%. Robustness of the model was validated and blind test samples were correctly assigned to their respective cluster.
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Keywords
Chemometric Analysis Of Amino Acids And Minerals Composition , For Traceability Of Edible Bird’s Nest