Profiling Of Triacylglycerols And Identification Of Markers In Edible Fats And Oils Using Liquid Chromatography Tandem Mass Spectrometry

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dc.contributor.authorSyed Idrus, Syed Izmuhar
dc.date.accessioned2020-06-12T08:48:08Z
dc.date.available2020-06-12T08:48:08Z
dc.date.issued2019-01
dc.description.abstractHalal certification and authentication has much been sought out globally especially in Malaysia that is govern by regulatory bodies such as Jabatan Kemajuan Agama Islam Malaysia (JAKIM). In order to achieve this, adulterants testing method has to be stringent so the identification of adulterant can be accurately analyzed. Target analyte for analysis has primarily been Deoxyribonucleic Acid (DNA) and testing that involved in DNA analysis such as Polymerase Chain Reaction (PCR). Therefore a new target analyte and techniques are needed to supplement this current testing analysis by using Reverse Phase Ultra High Liquid Chromatography tandem Mass Spectrometry (UHPLC-MS) targeting Triacylglycerols (TAGs). The main objectives of this study are to develop an optimization method for C18 core-shells columns and generate mas spectrometric profiling of TAGs in edible fat and oils and to apply the developed methods and techniques in detecting TAGs in food sample in identifying possible food markers. The column comparison calculation shows that C18 core-shell column has a resolution of 2.23, higher than the C18 porous column resolution of 0.91 at the same flow rate of 400 μl/min. The overall specifications of the C18 core-shell efficiency and peak capacity of 0.72 and 27.0 respectively did not deviate too much from the C18 porous while maintaining the high resolution of separation. The C18 core-shell has also separation corn oils that yield Oleic-Oleic-Linolenic that was not present in the C18 porous column.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9694
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectTriacylglycerolsen_US
dc.subjectChromatographyen_US
dc.titleProfiling Of Triacylglycerols And Identification Of Markers In Edible Fats And Oils Using Liquid Chromatography Tandem Mass Spectrometryen_US
dc.typeThesisen_US
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