Pharmacological Studies And Establishment Of Tissue Culture For Hymenocallis Litoralis
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Date
2012-06
Authors
Sundarasekar, Jeevandran
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Hymenocallis littoralis an ornamental plant exhibits numerous therapeutic properties such as anti-Candida, antioxidant, cytotoxicity and wound healing activities. Hymenocallis littoralis parts including anther, bulb, flower, leaves, stem and roots were extracted via sonication technique using methanol solvent. The wild plant extracts was subjected to anti-Candida activity and it shows promising activity against Candida albicans strain (ATCC 10231). The flower and root methanolic extracts exhibits better anti-Candida activity at 6.25 mg/mL as minimum inhibitory concentration (MIC). Scanning and transmission electron microscopy analysis reveals the cells lost its cell wall contact integrity and also nucleus properties at 36 h of treatment. The flower and anther methanolic extracts shows ferric reducing antioxidant power (FRAP) at 555.12 ± 1.67 and 568.09 ± 0.42 μmol (Fe2+)/g. Beside FRAP, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay carried out and it expresses inhibitory concentration at 50 % (IC50) for flower: 1.29 mg/mL, leaves: 5.07 mg/mL, stem: 1.33 mg/mL, bulb: 5.42 mg/mL, root: 3.68 mg/mL and anther 0.31 mg/mL. Finally, the phenolic and flavonoid contents of all the extracts were analyzed. The phenolic content in ascending order was root < leaves < stem < bulb < flower < anther, while flavonoid content was bulb < root < leaves < flower < stem < anther. In conclusion, the methanolic extract of anther exhibits better antioxidant activity with high amount of phenolic and flavonoid contents. Brine shrimp lethality assay was carried out to determine the cytotoxicity activity and the methanolic
leaves extract exhibits 100 % death for all the nauplii at 12 h of treatment point.
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Keywords
Pharmacological studies and establishment of tissue culture , for Hymenocallis litoralis