In Vitro Antioxidant, Antiangiogenic And Vasorelaxant Studies Of Clerodendron Serratum (Spreng.) Leaves Extracts
Loading...
Date
2011-06
Authors
Mohamed, Ali Jimale
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Clerodendron serratum (Spreng.) leaves from Verbenacea family, locally known as “Timba Tasek” is a traditional Malay medicinal plant used for treating various diseases. However, the present works were focused on evaluating its antiangiogenic, vasorelaxant and antioxidant activities as well as chemical profiles of the leaves extracts. The dried powdered leaves of C. serratum were serially macerated with petroleum ether (a defatting step), chloroform, methanol and water. The antiangiogenic activity (the inhibitory effects of these extracts on blood vessel growth/formation) was studied using rat aortic ring assay. The vasorelaxant activity was studied by examining the inhibitory effect of C. serratum extract on contractile responses of cumulative doses of noradrenaline in isolated rat aortic ring preparation. The antioxidant activity was determine using DPPH• radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) assays. It was found that amongst the extracts, the methanolic extract (ME-CS) showed the most potent antiangiogenic, vasorelaxant and antioxidant activities. Therefore, the methanolic extract was fractionated using n-hexane, chloroform, ethyl acetate, n-butanol and water. Amongst the fractions, the n-butanol fraction of ME-CS showed the most potent antiangiogenic, vasorelaxant and antioxidant activities. Phythochemical study revealed that n-butanol fraction contains relatively large amount of polyphenolics compounds and some terpenoids and saponins. Since, all three activities (antioxidant, antiangiogenic and vasorelaxant) end up in the same fraction, it is very likely that all the activities were contributed by the same group of chemical compounds. The antioxidant activity may be involved in its antiangiogenic and vasorelaxant activities of C. serratum leaves.
Description
Keywords
Clerodendron serratum a traditional Malay medicinal plant , used for treating various diseases