Synthesis, Characterization And Properties Of Chitosan-Poly (Methacrylic Acid-Co-N-Isopropylacrylamide) Hydrogel For Drug Delivery
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Date
2019-05-01
Authors
Md Rasib, Siti Zalifah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The application of chitosan has received enormous attention in medical field
particularly in drug delivery. In this study, chitosan with two monomers, methacrylic
acid (MAA) and N-isopropylacrylamide (NIPAM) were prepared by emulsion
polymerization to form a chitosan-poly(methacrylic acid-co-N-isopropylacrylamide)
(chitosan-p(MAA-co-NIPAM)) hydrogel as a drug carrier. Hydrogels were
characterized using Fourier Transform Infrared (FTIR) Spectroscopy, Carbon-13
Nuclear Magnetic Resonance (13C-NMR), Field Emission Scanning Electron
Microscope (FESEM), particle size analysis and Dynamic Light Scattering (DLS).
The effect of stimuli responsive (pH and temperature), hydrolysis, drug loading, in-vitro drug release and cytotoxicity was investigated. The finding found that chitosan-p(MAA-co-NIPAM) hydrogel synthesized using surfactant (Span 80) successfully
achieved particle size less than 500 nm. The cationic surface charge of synthesis
hydrogel passed the stability requirement to avoid aggregation in blood system above
30 mV at pH 3 to 5 and potentially enhanced cellular internalization drug delivery up
to pH 6. The release of Rifampicin drug was controlled by combination of diffusion
and swelling as the drug released was prolonged to 72 hours indicating that the
hydrogel responded to the pH and temperature environment. High viability cell from
cytotoxicity assessment demonstrated that the hydrogels are human/animal friendly
biomaterials. Thus, it can be concluded that chitosan-p(MAA-co-NIPAM) hydrogels
are feasible as controlled drug delivery carrier.