Vancomycin And Its Beta-Cyclodextrin Complex : Physicochemical Characterization And Its Cytotoxicity Evaluation Of Human Glial Cell Line

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dc.contributor.authorSofian, Zarif Mohamed
dc.date.accessioned2018-12-18T07:08:45Z
dc.date.available2018-12-18T07:08:45Z
dc.date.issued2011-06
dc.description.abstractIn this present study, the equimolar complexes of vancomycin, a broad-spectrum glycopeptide antibiotic with β-CD were prepared to evaluate their suitability for the development of a prolonged-release form of the antibiotic. β-CD/vancomycin complexes were prepared accordingly via freeze-drying and kneading techniques. The formation of the complexes was confirmed through X-ray Diffractometry (XRD), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM) and Fourier Transfrom Infrared Spectroscopy (FT-IR). The results obtained were all in good agreement suggesting the successive formation of the aforementioned binary systems. The XRD diffractograms of the complexes showed marked reduction in crystalinity which indicates the presence of vancomycin in the complexes. In the SEM microimages captured, significant difference in surface morphology was observed between the complexes and the starting materials. Distinct thermal profiles between the complexes and the pure molecules recorded signify the existence of possible reciprocal interactions between the two molecules. Spectral variations included broadening, shifting and reduction in relative intensities of the absorption bands were also observed in the IR spectra of both complexes. Apart from that, the disappearance of guest signals in the complexes spectra can be considered as a confirmation for the formation of β-CD /vancomycin complexes. However, from the experiments it was found that the kneaded complex was more efficient in prolonging the delivery of vancomycin compared to the freeze-dried complex. The possible cytotoxic effects of vancomycin and its complex with β-CD on human glial cell were assayed by means of MTS assay.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7335
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThe equimolar complexes of vancomycinen_US
dc.subjecta broad-spectrum glycopeptide antibioticen_US
dc.titleVancomycin And Its Beta-Cyclodextrin Complex : Physicochemical Characterization And Its Cytotoxicity Evaluation Of Human Glial Cell Lineen_US
dc.typeThesisen_US
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