The Use Of Water- And Pocket-Pulse Amplitude Modulation In Investigating Photophysiology And Nutrient Limitations In Microalgae

dc.contributor.authorMuhamad Darif, Nur Aqilah
dc.date.accessioned2019-04-17T01:53:03Z
dc.date.available2019-04-17T01:53:03Z
dc.date.issued2018-05
dc.description.abstractInvestigating photosynthesis and nutrient limitations of microalgae allow for insight into how cells will respond to different environmental or physical stressors particularly nutrients. Advances in chlorophyll fluorescence techniques have provided a means for rapid and non-invasive measurement of microalgal ecophysiology. Furthermore, available Pulse Amplitude Modulation (PAM) fluorometers in the market present different technical characteristics and configuration which may lead to different photosynthetic parameters output. Here the photosynthetic activities of microalgae were observed using both Water-PAM and Pocket-PAM to validate the differences between these two fluorometers. Microalgal community samples were collected from Teluk Bahang and Pantai Jerejak while single species laboratory cultures were obtained from the Centre for Marine and Coastal Studies (CEMACS), Penang. For both field and laboratory studies, maximum quantum yield (Fv/Fm), maximum relative electron transport rate (rETRmax) and photoacclimation index (Ek) of Water-PAM were significantly different (P < 0.05), about 50% to those of Pocket- PAM. For example, in Pantai Jerejak community sample, photosynthetic rates (rETRmax) increased by four-fold when using Water-PAM (67.5 ± 3.7) by comparison to Pocket-PAM (16.6 ± 2.1). Similarly, nutrient-induced fluorescence transient (NIFT) using Water-PAM and Pocket-PAM were different in terms of the response output. This study showed that care should be taken when interpreting results using different PAM fluorometers because they are different in terms of technical characteristics such as approximate detection limit, configuration and actinic light colour. To further understand the effect of silica on photosynthesis of microalgae, silica concentrations were manipulated in two species of diatoms: tropical Actinocyclus octonarius and polar Chaetoceros neglectus.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8025
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectInvestigating photosynthesis and nutrient limitationsen_US
dc.subjecthow cells will respond to different environmentalen_US
dc.titleThe Use Of Water- And Pocket-Pulse Amplitude Modulation In Investigating Photophysiology And Nutrient Limitations In Microalgaeen_US
dc.typeThesisen_US
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