Impact Of Pressurized Extraction And Electrohydrodynamic Encapsulation Methods On Antioxidative Properties Of Momordica Charantia Fruit Extract
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Date
2018-04
Authors
Torkamani, Amirehsan
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The objectives of this study were first to model pressure assisted extraction of
polyphenolic antioxidants from bitter gourd (BG) as a model fruit-like vegetable and
secondly to characterize electrohydrodynamic nanoencapsulation methods within natural
polymeric matrices. Response surface methodology (RSM) was used to investigate and
optimize the impact of extraction time, temperature and induced calorimetric power on
total phenolic content (TPC), total flavonoid content (TFC), Ferric reducing/antioxidant
power assay (FRAP) and Radical scavenging activity (RSA) of BG ultrasonic assisted
extract (UAE). The optimal extraction condition was reached at 52.00 W of induced
power, extraction time of 20 min and temperature of 60 °C. Furthermore, statistical
analysis revealed that antioxidative attributes of extracts were correlated with the effect
of power input and temperature rather than processing time. Pressurized liquid extraction
(PLE) of antioxidant compounds from BG fruits in the aqueous ethanolic solvent was
investigated using RSM at a laboratory scale. Extraction efficiency was optimized by
measuring the TPC, TFC, FRAP and RSA. The optimal extraction conditions were
reached at 80% ethanol concentration, 10 min extraction time and at 160 °C of
temperature. Statistical analysis revealed that antioxidative attributes of bitter gourd
extract were strongly (P<0.05) correlated with positive extraction temperature and
ethanol concentration effect rather than processing time. These studies illustrated that
UAE and PLE both have potential to extract antioxidant compounds from tropical fruit
vegetables in an accelerated manner. However, PLE demonstrated higher efficacy in
terms of yield and required processing time. Electrospraying process was conducted at 20
kV, 0.5 mL/ h and 10 cm of voltage, flow rate and emitter/collector distance, respectively
and were encapsulated with bitter gourd extract (BGE) at 5 to 15% (w/w%) rate.
Description
Keywords
Impact of pressurized extraction , antioxidative properties of momordica charantia