Impact Of Pressurized Extraction And Electrohydrodynamic Encapsulation Methods On Antioxidative Properties Of Momordica Charantia Fruit Extract

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Date
2018-04
Authors
Torkamani, Amirehsan
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Publisher
Universiti Sains Malaysia
Abstract
The objectives of this study were first to model pressure assisted extraction of polyphenolic antioxidants from bitter gourd (BG) as a model fruit-like vegetable and secondly to characterize electrohydrodynamic nanoencapsulation methods within natural polymeric matrices. Response surface methodology (RSM) was used to investigate and optimize the impact of extraction time, temperature and induced calorimetric power on total phenolic content (TPC), total flavonoid content (TFC), Ferric reducing/antioxidant power assay (FRAP) and Radical scavenging activity (RSA) of BG ultrasonic assisted extract (UAE). The optimal extraction condition was reached at 52.00 W of induced power, extraction time of 20 min and temperature of 60 °C. Furthermore, statistical analysis revealed that antioxidative attributes of extracts were correlated with the effect of power input and temperature rather than processing time. Pressurized liquid extraction (PLE) of antioxidant compounds from BG fruits in the aqueous ethanolic solvent was investigated using RSM at a laboratory scale. Extraction efficiency was optimized by measuring the TPC, TFC, FRAP and RSA. The optimal extraction conditions were reached at 80% ethanol concentration, 10 min extraction time and at 160 °C of temperature. Statistical analysis revealed that antioxidative attributes of bitter gourd extract were strongly (P<0.05) correlated with positive extraction temperature and ethanol concentration effect rather than processing time. These studies illustrated that UAE and PLE both have potential to extract antioxidant compounds from tropical fruit vegetables in an accelerated manner. However, PLE demonstrated higher efficacy in terms of yield and required processing time. Electrospraying process was conducted at 20 kV, 0.5 mL/ h and 10 cm of voltage, flow rate and emitter/collector distance, respectively and were encapsulated with bitter gourd extract (BGE) at 5 to 15% (w/w%) rate.
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Keywords
Impact of pressurized extraction , antioxidative properties of momordica charantia
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