Cloning and characterization of mycobacterium tuberculosis katG gene
Loading...
Date
2005
Authors
Martin, Eugene Ray
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
Abstract
katG is tbe sole gene encoding for catalase peroxidase enzyme in
Mycobacterium tuberculosis enabling its survival in the host macrophage. This same
gene has been indicted to play an important role in forming the killing effect of
isoniazid (INH), a major drug used in the treabnent of tuberculosis (TB)~ It has been
indicated that the folDlation of new generation drugs for TB will be connected to
further understanding this interaction and the study of the structures of the katG
gene.
The high molecular weight genomic DNA of Mycobacterium tubercuJ.o.,;., of
the clinical strain MTB69/03 from HUSM has successfully been isolated tbroogb a
modification of the method in D. van Soolingen et. al .. Two primers with extra sites
for tbe excision sites of EcoRl and Hind/11 were successfully used to produce a
DNA product of 2252 base pairs. The product was then inserted into a pCR®.2.lTOPO
® plasmid vector. The insert was continued by the use of both PCR and
restriction enzymes.
Tite successfully confinned inserted plasmids were then sent for sequencing.
Description
Keywords
tuberculosis