Field evaluation of a potential infection marker for Burgian filariasis

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dc.contributor.authorNoordin, Rahmah
dc.contributor.authorRiazi, Mehdi
dc.contributor.authorAzahari, Noor A'shikin
dc.contributor.authorHuat, Lim Boon
dc.contributor.authorBujang, Abdullah
dc.contributor.authorNoor, Zurainee Mohd
dc.contributor.authorAnuar, Khairul
dc.date.accessioned2018-10-25T01:18:09Z
dc.date.available2018-10-25T01:18:09Z
dc.date.issued1999
dc.description.abstractWestern blot analyses were performed on 444 serum specimens : 40 sera from microfilaraemic individuals,10 sera from elephantiasis patients, 24 treated individuals, 50 sera from residents of endemic areas without anti-filarial IgG4 "antibodies (endemic normals), 20 sera from amicrofilaraemic individuals with high anti-filarial lgG4 antibodies, 200 sera from healthy city-dwellers (non-endemic samples) and 100 sera from soil-transmitted helminth infected individuals. Phast Electrophoresis System was used to electrophorese Brugia malayi soluble adult worm antigen on 10-15% SDS-PAGE gradient gels followed by electrophoretic transfer onto PVDF membranes. Membrane strips were then successively incubated with blocking solution, human sera and monoclonal anti-human IgG4 antibody-HRP ; with adequate washings done in between each incubation steps. Luminol chemiluminescence detection was then used to develop the blots. An antigenic band with the - MW of 37 kDa was found to be consistently present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with high titres of anti-filarial IgG4 antibodies, some treated patients and some elephantiasis patients. The antigen did not occur in immunoblots of individuals with other helminthic infections, normal endemic individuals and city dwellers. Therefore the B. malayi antigen of- MW of 3'7 kDa demonstrated specific reactions with sera of B. malayi infected individuals and thus may be useful for diagnostic application.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6875
dc.language.isoenen_US
dc.publisherKampus Kesihatan, Universiti Sains Malaysiaen_US
dc.subjectBrugia Malayien_US
dc.subjectSpecific antigenen_US
dc.titleField evaluation of a potential infection marker for Burgian filariasisen_US
dc.typeWorking Paperen_US
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