Single Nucleotide Polymorphisms (SNPs) Of Adiponectin Gene And Its Association With Serum Adiponectin Concentration And Metabolic Syndrome Risk Factors Among Malays Adults
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Date
2011-08
Authors
Isa, Nur Firdaus
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Publisher
Universiti Sains Malaysia
Abstract
Metabolic syndrome is a cluster of risk factors that include central obesity, hypertriglyceridaemia, reduced HDL cholesterol, hypertension, and hyperglycemia. Accumulating evidences support the hypothesis that hypoadiponectinemia, a type of adipokine, confer increased risk for metabolic diseases. Of interest, some of the common polymorphisms in the promoter region, exon and intron 2 of the human adiponectin gene are associated with risk factors of metabolic syndrome. The present study aims to investigate the association of several single nucleotide polymorphisms in the adiponectin gene with serum adiponectin concentration and metabolic syndrome risk factors among Malay adults. A total of 298 Malay adults were recruited in this study. Measurements for waist circumference and blood pressure were taken before drawing an overnight fasting blood. Biochemical tests for triglycerides, HDL cholesterol and glucose were carried out by using commercially available kits. Plasma adiponectin concentration was measured using Human Adiponectin ELISA kit. A total of five sites of single nucleotide polymorphisms in adiponectin gene (SNPs 11426, -11391 and -11377 at proximal promoter and SNPs +276 and +45 at exon 2 regions) were screened using minisequencing method. Findings from this study showed that the adiponectin concentration in the subjects with MS was significantly lower than those without MS (p < 0.05). The adiponectin concentration was also significantly associated with only hypertriglyceridemia (p < 0.001) and reduced HDL cholesterol (p < 0.001). None of the studied SNPs or haplotypes showed any significant association with the adiponectin concentration.
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Keywords
Metabolic syndrome is a cluster of risk factors , that include central obesity