Biologi pembiakan dan pembenihan sefalopoda, sepioteuthis lessoniana (lesson) dengan kaedah separa intensif

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Date
1998-01
Authors
Othman, Mohd.Fariduddin
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Abstract
A study was conducted on big fin reef squid, Sepioteuthis lessoniana to understand its reproductive biology in the wild, egg transportation methods to the hatchery, incubation and hatching procedures, and breeding requirements. A survey of the breeding population and breeding ground was conducted and analyzed from squid trap operations ati.d catch. landings data. Semi-intensive breeding trials and related experiments were conducted at the marine shrimp hatchery of the National Prawn Fry Research and Production Centre (NAPFRE), Fisheries Department, Kg. Pulau Sayak, Kedah. In the wild, spawning activities were seasonal and localized. Breeding season was between October-January. The breeding ground was about 2-10 nautical miles offshore, at 5-10 m depth with salinity of 30-33 ppt, pH of 8.3-8.6, water temperature of 26-31 °C, dissolved oxygen of 4.7-7.2 ppm, turbidity of2.5-7.5 m and water current of 0.0-0.2 tn!s. Matured males weighed 630 ± 330 g (body weight - BW) and more than one individuals patticipated in mating and copulation activities with a single female. Gravid females weighed 400 ± 170 g (BW) with an average ovary size of 29.0 ± 16.6 g and fecundity of 123-14 70 . oocytes per female. The fecundity was directly related (R2 = 0.5609 ) to size (mantle length - ML). The ovarian oocytes.(total length - TL 0.58 ± 0.08 em, width- Wd 0.40 ± 0.06 em, weight- Wt 0.04 ± 0.05 g) and laid eggs were physically and structurally different. The laid eggs were enclosed in a single, finger-like elongated jelly capsule (TL 3.5 ± 0.5 em, Wd 0.9 .± 0.2 em, W 3.1 ± 0.3 g ,) whereby each was linked, forming a single egg mass of mop-like structure of 300-450 capsules with an average weight of 900-1400 g. Individual capsule carried 3-7 (mean 4.2) embryos. Transportation of egg mass at any maturity stage from their natural habitat to the hatchery did not significantly effect (P>0.05) hatching rate. Incubation was done best by hanging or free floating method. After 18-21 days, egg capsules (TL 12.2 ± 0.3 em, W d 1.8 ± 0.2 em, W 12.7 ± 1.6 g,) hatched. The processlasted 6-8 days and peaked on day 3-5 with a hatching rate between 40-50 %. Incubation, hatching and larval rearing were optimal when turbidity was near zero with salinity of 28-31 ppt, minimum dissolved oxygen of3 ppm, pH of7.6-8.2 and water temperature of 28-30 °C. The juveniles resembled the adults in many aspects e'xcept for size (ML 0.4-0.5 em) and began feeding strictly on live food 1-2 days later,. Shrimp larvae (TL 0.5-1.3 em) were preferred. Adult Artemia (TL 0.5-1.2 em), fish larvae (TL 0.7-1.5 em) or Acetes (TL 0.5-1.3 em) were not well accepted till day 7-IO. After day 23-25 juveniles began to accept fresh fish flesh and gradually switched to dead fish. Larval rearing is practiced best in two phases to optimize feed distribution as well as to minimize cannibalism. TI1e juveniles (BW 7-11 g, ML 3.5-4.5 em) packed at the rate of 2.5- 6.5 individuals/! for an 8-h journey survived 100% on arrival. Except for the posthatching period, survival was consistent as growth proceeded. The marketable size of 200-400g was achievable after 3-4 months of culture. After 4-5 months female ovary was fully developed and ready to spawn again. To improve breeding success, problem of live food supply has to be addressed beside emphasizing on research to improve survival in the early phase and to determine an optimum culture environment for the grow-out phase.
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Keywords
Sefalopoda, sepioteuthis lessoniana , Kaedah separa intensif
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