Development Of Liquid Chromatographic Methods For The Determination Of Aflatoxins And Fumonisins In Food And Feeds
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Date
2012-07
Authors
Al-Debi, Wijdan Shakir Khayoon
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
New sample preparation methods (using multifunctional clean-up column (MFC) and micro-solid phase extraction (μ-SPE) for the high performance liquid chromatography (HPLC) determination of aflatoxins (AFs) B1, B2, G1 and G2 are described. For the MFC method, the samples were first extracted using acetonitrile:water (90:10) and further purified using a single step. Optimum conditions for the extraction and chromatographic separation (using fluorescence detector (FLD)) were investigated. After the optimization, the developed method was applied for the determination of AFs in forty two animal feeds samples comprising corn (16), soya bean meal (8), mixed meal (13), sunflower, and 1 sample each of wheat, canola, palm kernel, copra meals. A μ-SPE method followed by LC-MS/MS separation and detection was successfully developed and optimized for the determination of AFs in food. The optimum conditions were: sorbent material, C8; sorbent mass, 20 mg; extraction time, 90 min; stirring speed, 1000 rpm; sample volume, 10 mL; desorption solvent, acetonitrile; solvent volume, 350 μL and ultrsonication period, 25 min without salt addition. Under the optimum conditions, enrichment factor of 11, 9, 9 and 10 for AFG2, AFG1, AFB2 and AFB1, respectively were achieved. Good linearity and correlation coefficient was obtained over the concentration range of 0.5-50 ng g-1 (r2 0.9988 - 0.9999). The method was applied to analysis 20 samples of malt beverage (9) and canned coffee(11). No AFs were detected in the selected samples.
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Keywords
Development of liquid chromatographic methods , for the determination of aflatoxins