Determination Of R-3-Hydroxyacylacp- Coa Transferase (Phag) Structure
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Date
2010-03
Authors
Arsad, Hasni
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
R-3-hydroxyacyl-ACP-CoA transferase (PhaG) catalyzes the conversion of (R)-3-
hydroxyacyl-ACP to (R)-3-hydroxyacyl-CoA derivatives, which serve as the
ultimate precursor for polyhydroxyalkanoate (PHA) polymerization from unrelated
substrates. PHA is a family of bioplastic that has a good potential to replace fossilbased
thermoplastics because it is biodegradable. The transferase enzyme PhaG of a
locally isolated strain, Pseudomonas sp. USM 4-55, was recently cloned (GeneBank
accession number EU305558). Currently there is no known 3D structure with high
similarity to PhaG. In order to over express, the phaG gene was cloned into
expression vector pQE-30 and it was successfully overexpressed by induction with
0.5 mM IPTG in the host Escherichia coli strain SG 13009.
Description
Keywords
R-3-Hydroxyacylacp- Coa Transferase (Phag)