Desaturase Delta-6 Mrna Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio Rerio)

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dc.contributor.authorIshak, Sairatul Dahlianis
dc.date.accessioned2018-11-05T06:40:26Z
dc.date.available2018-11-05T06:40:26Z
dc.date.issued2010-06
dc.description.abstractVery little is known about the synthesis of unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), or collectively known as the highly unsaturated fatty acids (HUFA), in the fish ovary although they play pivotal roles in this vertebrate reproduction. The zebrafish (Danio rerio) display capability to synthesize all three HUFA via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, C18:3n-3). In order to gain understanding on the importance and regulation of ovarian HUFA synthesis, this study shows the mRNA expression pattern of desaturase (fadsd6), an essential enzyme involved in HUFA biosynthesis pathway, in five different zebrafish ovarian follicle stages; pre-vitellogenic, early vitellogenic, late vitellogenic, maturation and ovulation stages. The mRNA levels of fadsd6 in different ovarian follicle stages were determined by semi-quantitative RT-PCR assays.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6998
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectDesaturase Delta-6 Mrna Expressionsen_US
dc.subjectFollicle Maturation In Female Zebrafish (Danio Rerio)en_US
dc.titleDesaturase Delta-6 Mrna Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio Rerio)en_US
dc.typeThesisen_US
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