Development Of Effective Spore Induction Methods For Asporogenic Fungal Species Using The Standard Culture Medium
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Date
2018-04
Authors
Tan, Kathrine Xin Yee
Journal Title
Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Factors enhancing anamorph spore production were challenged. Effects of types of media and lighting conditions in the induction of Cercospora citrullina Cooke conidiation as well as effects of banana leaf-piece agar (BLA) in the induction of Fusarium spp. macroconidia production were studied. C. citrullina isolated from pumpkin leaves with Cercospora leaf spot disease (CLS) was found unable to produce conidia when cultured on potato dextrose agar. Pathogenicity test was carried out on several cucurbit host plants prior to the study on effect of culture medium and substrates under different lighting conditions in inducing the conidiation of C. citrullina. Commercially available oatmeal agar; homemade agar prepared from the infusion of cereal grains such as oat grain, wheat grain and barley grain in addition to substrates prepared from dried Trichosanthes sp. (Cucurbitaceae) leaves, oat grain, wheat grain and barley grain were effective in inducing conidia production in C. citrullina (p = 0.05). Conidia production was prominently found when the C. citrullina cultures were incubated under 12 h fluorescent white light irradiation plus 12 h darkness and complete darkness condition (p = 0.05). A total of six isolates of Fusarium were cultured on six types of banana leaf-piece agar (BLA) prepared from the leaves of Musa × paradisiaca (Nangka and Tanduk), Musa acuminate × balbisiana Colla ‘Raja’ (Toman and Talun), M. acuminata × balbisiana cv. Awak and M. acuminata spp. malaccensis (Wild) respectively to learn about the effects of BLA in the macroconidia production of Fusarium. Comparisons of shape and size of
macroconidia produced on different types of BLA with standard macroconidia inducing medium, carnation leaf- piece agar (CLA) were made.
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Keywords
Effective spore induction methods , asporogenic fungal species