Evaluation Of Burkholderia Sp. Usm (Jcm 15050) For The Production Of Copolymeric Polyhydroxyalkanoate Containing 3-Hydroxy-4- Methylvalerate Monomer
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Date
2013-12
Authors
Lau, Nyok Sean
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The nutrition-versatility of Burkholderia sp. strain USM (JCM 15050) has
initiated the studies on the use of this bacterium for polyhydroxyalkanoate (PHA)
production. This study was carried out to evaluate the ability of Burkholderia sp.
USM (JCM 15050) and its transformant harboring the PHA synthase gene of
Aeromonas caviae to incorporate a new type of PHA monomer, 3-hydroxy-4-
methylvalerate (3H4MV). This monomer with methyl side chain is of interest since
the monomer can prevent the deterioration of poly(3-hydroxybutyrate-co-3-hydroxy-
4-methylvalerate), P(3HB-co-3H4MV) during aging. To promote the accumulation
of PHA containing 3H4MV monomer, 4-methylvaleric acid was provided as cocarbon
source. The polymerization of 3H4MV monomer by Burkholderia sp. USM
(JCM 15050) confirmed the ability of Burkholderia sp. USM (JCM 15050) synthase
to accept CoA thioester of 3H4MV, a six-carbon atom monomer, as substrate.
Various culture parameters such as concentrations of NR medium, fructose and 4-
methylvaleric acid as well as harvesting time were manipulated to produce P(3HBco-
3H4MV) with different 3H4MV compositions. The structural properties of PHA
containing 3H4MV monomer were investigated by using nuclear magnetic resonance
(NMR) and fourier transform infrared spectroscopy (FTIR). The relative intensities
of the bands at 1183 and 1228 cm-1 in the FTIR spectra enabled the rapid detection
and differentiation of P(3HB-co-3H4MV) from other types of PHA.
Description
Keywords
Burkholderia Sp. Usm (Jcm 15050) , Copolymeric Polyhydroxyalkanoate