Cytotoxicity Of Clinacanthus Nutans Methanol-Water Extract On Huh-7 Cell Line
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Date
2017-08
Authors
Chuah, Jia Jun
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
There are many of cancer cases reported yearly and it has been increasing as day passes by. Many sought out the capabilities of natural herbs instead of relying on the common treatment currently being used for cancer which are chemotherapy and radiotherapy. One of the popular plants being used currently is Clinacanthus nutans or commonly called ‘belalai gajah’ by local Malaysians. The objective of this research is to determine the cytotoxicity effects of the methanol-water extract of this plant on Huh-7 cell lines, a well differentiated and well-established hepato cellular carcinoma taken from the liver of a 57 year old Japanese male. The cells obtained from JCRB, Japan is cultured first according to protocol before seeding into 96-well plates. Then, the prepared methanol-water extract is then added into each well which was seeded and is treated for time point 24 hours, 48 hours and 72 hours with concentrations of 1 mg, 2 mg, 3 mg, 4 mg, 5mg, 6 mg, 7 mg, 8 mg, 9 mg and 10 mg. The cell viability determination is measured by wavelength absorbance through the use of MTT and SRB reagent. The results showed that the extract does exhibit cell proliferation inhibiting properties but is not effective at 4 mg dose after 24 hours and at 72 hours, there is some significant inhibition. However, it is very effective at 10 mg dose after 24 hours as the percentage of cell viability is very low. The methanol-water extract of Clinacanthus nutans does shows cell proliferation inhibiting properties however only applicable at higher doses in Huh-7 cell line.
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Keywords
Cytotoxicity of Clinacanthus nutans , Methanol-Water Extract on Huh-7 Cell Line.