The Effect Of Goniothalamus umbrosus Extracts On Hela Cell
Loading...
Date
2015
Authors
Wan Aziz, Wan Nuraini
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Cervical cancer is the fourth leading cause of cancer death in female worldwide. The rate of incidence and mortality is higher in developing countries including Malaysia. Although the survival of the patients had increased due to the rapid advancement of neoadjuvant chemotherapy and surgery or both, but there still results in substantial morbidity. Moreover, most of the cases for current treatments do not show improvement and results side effects to the patients. Currently, chemoprevention approach using natural products is one of great interact among researchers. This study evaluated the potentially of the Goniothalamus umbrosus (Kenerak) leaves extracts in exhibiting chemopreventative effects on cervical cancer cell (HeLa cells). The IC50 of the extracts were determined with MTS and AlamarBlue assay lies in 45.31 μg/ml and 48.44 μg/ml respectively. Morphological changes displaying apoptosis can be observed upon treatment with G. umbrosus leaves extracts, including cell shrinkage, rounding of cells, vacuolization and cell elongation. The 3 days cell proliferation assay indicated that G. umbrosus leaves extracts has an anti-proliferative effects. Overall, the data collected provide new insight of using G. umbrosus leaves extracts which can be used as chemopreventive agent on cervical cancer.
Description
Keywords
The effect of Goniothalamus umbrosus extracts , on hela cell