Anticandidal, Antioxcidant, Toxicity Studies And Isolation Of Compounds From Cassia Fistula Seeds
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Date
2011-10
Authors
Subramanion, Jo Thy Lachumy
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
In the present study, methanol seeds extract of Cassia fistula was assayed for antimicrobial and antioxidant activities, isolated and purified for bioactive compounds as well as evaluated for the toxicity effect. The seeds extract was tested for potential antimicrobial activity against medically important Gram-positive and Gram-negative bacteria and fungal strains. The antimicrobial activity was determined by the disc diffusion and the broth dilution methods. The extract was effective on tested microorganism and the minimum inhibitory concentration (MIC) values were found in the range of 1.563-50.00 mg/mL. The seeds extract showed a favourable activity against pathogenic fungi C. albicans with MIC value 6.25 mg/mL, and this was confirmed by time kill assay study. The effect of C. fistula seeds extract on C. albican was examined by the alteration in normal growth profile curve and this further confirms the anticandidal activity on C. albicans. The SEM and TEM observations were carried out to distinguish the metamorphosis in the morphology of control and seeds extract treated C. albicans. The results revealed adverse effects on the outer cell wall and cytoplasmic content of the C. albicans and complete collapsed of the yeast cell exposed to seeds extract at concentration 6.25 mg/mL for 36 hrs. The phytochemical screening analysis of C. fistula seeds extract revealed the presence of five major secondary metabolites including anthraquinone, terpenoids, flavonoids, saponins, tannins and the absence of alkaloids and reducing sugars. Hence, further purification and identification of bioactive compounds were done by various chromatography techniques. Two separated bands were separated
from the developed chromatogram with Rf value 0.39 and 0.69. The isolated active fractions were tested via HPLC and LC/MS, and the results revealed distinct peaks which indicated the presence of major bioactive compound with anticandidal activity.
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Keywords
Isolation of compounds from , Cassia fistula seeds