Detection Of Hpv-Dna In Cervical And Urethral Scrapings Using Hybrid Capture 2 And Nested-Pcr And Their Correlation With Cytomorphology
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Date
2010-05
Authors
Othman, Norodiyah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Human papillomavirus (HPV) infection is a causative agent for development of cervical
cancer. This study aims to detect the presence of HPV in routine pap smears of women
who attended Hospital Kota Bharu, Hospital Universiti Sains Malaysia and Hospital
Kuala Terengganu. The cervical sample from each woman was in two forms (split
sampling); conventional smear (CP) and ThinPrep® smear (TP). These women were
given a questionnaire to determine the risk factors for cervical cancer development. The
male spouses were invited to provide urethral scrapings for HPV studies. The cervical
and urethral samples were subjected to Hybrid Capture 2 and nested-PCR. HPV results
of the cervical scrapings were correlated with cytological diagnosis from CP and TP.
A total of 705 cervical scrapings and 99 male urethral scrapings were collected. 702
cervical samples were available for both CP and TP cytological evaluation. By CP,
92.7% of the collected cervical scrapings had normal smear, 2.0% had abnormal smear
and 5.3% smears were unsatisfactory for evaluation. By TP, 87.1% was normal, 1.7%
was abnormal and 11.1% was unsatisfactory for evaluation. The diagnoses of the
conventional Pap smears and ThinPrep® Pap smears were 88.9% in agreement. The
adequacy of sampling by TP (88.9%) is comparable to CP (94.7%).
635 cervical samples were sufficient for HPV testing. 44 (6.9%) samples were positive
for HPV; 27 by HR-HC2 and 28 by nested-PCR. The latter is more sensitive to detect
HPV; 72.7% of sensitivity compared to HR-HC2; 36.4% sensitivity while the specificity
is fairly similar; 96.8% and 96.3%. HPV detection improved when cytological diagnosis
from ThinPrep® smear (TP) was used as gold standard. The genotypes of the 21/28
(75.0%) nested-PCR HPV positive samples were; HPV-16 (57.1%), HPV-58 (19.0%),
HPV-6 (9.5%) and HPV-18, -33 and -61 (4.8%).
Description
Keywords
Detect the presence of HPV in routine pap smears , of women who attended Hospital Kota Bharu