Enhanced Production Of Lignin Peroxidase And Manganese Peroxidase By Phanerochaete Chrysosporium In A Submerged Culture Fermentation And Their Application In Decolourisation Of Dyes
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Date
2008-01
Authors
Chai, Chu Chia
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Abstract
The main problem when treating industrial wastewater is the removal of dyes.
Some of the physical and chemical treatment techniques were effective but they might
result in the production of toxic by-products and could also be the cause of a secondary
pollution problem due to excessive chemical use. In order to overcome the drawbacks,
the present work has focused on the biodegradation of dyes using lignin peroxidase
(liP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium. The study
was started with the optimization of enzymes production in the laboratory scale of
submerged system. In the study of producing MnP only, the optimization of some
governing parameters resulted in an increment of MnP about 175%. The optimal yield
of MnP (3.00 mU/ml) was found to be produced under the conditions of 0.2% (w/v) of
D-xylose, 0.4% (w/v) yeast extract, 2.0 mM ammonium dihydrogenphosphate, 1.0 mM
of MnS04, 1.0% (v/v) of 6x106 spore/ml as inoculum, initial pH of 4.0, cultivation
temperature at room temperature (28 ± 2°C) and agitation speed at 100 rpm. On the
other hand, in the study for optimization of liP production only, there was an increment
about 183%, and the optimal yield of liP (4.30 mU/ml) was produced under the
conditions of 0.1 % (w/v) of sucrose, 0.6% (w/v) yeast extract, 2.0 mM ammonium
dihydrogenphosphate, 0.4 mM of veratryl alcohol, 1.0% (v/v) of '6x106 sporelml as
inoculum, initial pH of 4.5, cultivation temperature at room temperature (28 ± 2°C) and
agitation speed at 150 rpm. In the study of optimizing MnP and liP in combination, the
optimal yield of MnP (4.64 mU/ml) and liP (5.37 mU/ml) resulted in an increment
approximately 744% and 253%, respectively, and the optimized conditions were 0.1 %
(w/v) of O-glucose, 0:6% (w/v) yeast extract, 2.0 mM ammonium dihydrogenphosphate,
0.4 mM of veratryl alcohol, 1.0 mM MnS04, 1.0% (v/v) of 6x106 sporelml as inoculum,
initial pH of 4.5, cultivation temperature at room temperature (28 ± 2°C) and agitation
speed at 150 rpm. As for the decolourisation of methylene blue, it could be occurred
through two stages, which were adsorption on the fungal biomass orland enzymatic
biodegradation. The medium with mixture of enzymes, in which both liP and MnP were
secreted together, achieved the highest decolourisation rate (87%), followed by liP
(82%) and MnP (57%) when the enzyme was produced separately. Therefore, the
production of liP and MnP in combination provided the most powerful effect of
decolourisation of methylene blue compared to the production of each enzyme
separately.
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Keywords
Biodegradation of dyes , using lignin peroxidase