Sebatian antibiotik daripada tanin yang oiekstrak daripaoa kulit pokok bakau minyak, rhizophora apiculata blume
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Date
2004
Authors
Sheh Hong, Lim
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Abstract
A research was conducted to study the antimicrobial effects of tannin from barks of
bakau minyak, Rhizophora apiculata Blume. Three types of tannin had been
extracted from barks of Rhizophora Apiculata Blume, namely mixed tannin,
hydrolysable tannin and condensed tannin. Screening using with disc diffusion assay
showed that the hydrolysable tannin of Rhizophora· apiculata Blume was more
effective in antimicrobial activity compared to mixed and condensed tannins.
In this study, special attention was given to hydrolysable tannin of Rhizophora
apiculata Blume in order to find out its ability as antimicrobial agent on tested
microorganisms.
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The results of me. study revealed that hydrolysable tannin of
Rhizophora apiculata Blume exhibited significant antibacterial and antiyeast activities
but not antifungal activity. The minimum inhibitory concentration, (MIC) and the
minimum lethal concentration (MLC), for hydrolysable tannin of Rhizophora
apiculata Blume had been determined for the selected test microorganisms. The MIC
value for hydrolysable tannin of Rhizophora apiculata Blume was 6.25 mglml and
minimum yeastocidal concentration (MYC) value was 12.5 mg/mt for Candida
albicans. The hydrolysable tannin of Rhizophora apiculata Blume at halfMIC, MIC
and two times MIC concentrations can inhibit the log phase of the yeast growth,
Candida albicans.
11vlicroscopic studies showed that hydrolysabk tannin of Rhi::.ophoro apiculma Blume
causes some morpholot.'Y changes in the treated cells, ( ·allllidaalhiccms. In ( ·cmdiclo
albicans cells, it causes abnonnal budding and dcfom1ation of the cell wall.
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The cytotoxicity test for hydrolysable tannin of Uhi::.oplwra apiculata Blume was
carried out using brine shrimp lethality assay and HepG2 cancer cells. 1t was found
that hydrolysable tannin of Rhi::ophora apiculato Blume showed significant values of
lethality concentration of 50% (LC;n). The hydrolysable tannin which extracted from
Rhizophora apiculata Blume also shows sib'llificant cytotoxicity effects on the HepG2
cancer cells. It was found that the number of survival HepG2 cancer cells became
less as the concentration of the hydrolysable tannin of Rhizophora apiculata Blume
increased.
The separation of the compounds of the hydrolysable tannin of Rhi::ophora apiculata
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Bh.une was done by using thin layer and column chromatography techniques. The
antiyeast activity test was performed afterwards. The antagonism effects were shown
by the hydrolysable tannin of Rhizophora apiculata Blume. This confinned since the
separating components of the hydrolysable tannin of Rhi::ophora apiculata Blume
showed a significant effect compared to the hydrolysable tannin. Through the highperformance
liquid chromatography (HPLC) method, the main component of the
hydrolysab1e tannin of Rhizophora apiculata Blume which react as antiyeast
(Candida albicans) was confirmed as gallic acid.
Description
Keywords
Kulit pokok bakau , Rhizophora apiculata blume