Complex Coacervated Microcapsules In Cream For Topical Delivery Of The Curcuminoids And Quercetin

Simple item page
dc.contributor.authorAng, Lee Fung
dc.date.accessioned2016-09-22T07:09:31Z
dc.date.available2016-09-22T07:09:31Z
dc.date.issued2015-12
dc.description.abstractThe curcuminoids and quercetin possess antioxidant, anti-inflammatory and antibacterial activities which are beneficial for wound healing. However, poor solubility, poor bioavailability, photosensitivity and color staining properties of these two compounds make them not acceptable for topical administrations. Therefore, the objectives of the present study were to develop and characterize the curcuminoids/quercetin microcapsules and subsequently incorporate them in cream. Isocratic reversed phase UV HPLC was developed and validated to quantify the curcuminoids (bisdemethoxycurcumin, demethoxycurcumin and curcumin) and quercetin simultaneously. The peaks of quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin were well separated and symmetrical. The microcapsules were prepared by complex coacervation method using chitosan and gelatin B. The best microcapsules were obtained at gelatin:chitosan ratio of 30:1 (2.55% w/w) and pH 5.50. The curcuminoids was encapsulated either in liquid (CLM) or solid (CPM) form. The quercetin was also encapsulated either in liquid (QLM) or solid (QPM) form. The physical characteristics of CPM and QPM were better than CLM and QLM. The CPM and QPM had particle size in the range of 40 – 44 μm, entrapment efficiency of 82% and drug loading of 16%. Moreover, the microcapsules had free flowing, reduce color staining effect and stable at room temperature (28 °C) for 12 months. The antioxidant activity of the curcuminoids and quercetin was higher than butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). There was no significant change in antioxidant activity of the curcuminoids and quercetin after encapsulation in microcapsules. The curcuminoids and quercetin microcapsules were then incorporated in O/W cream base, respectively. The preparations had pseudoplastic and thixotropic properties. In vitro release study revealed that the curcuminoids and quercetin released from microcapsule incorporated in cream followed zero order kinetics. The CPM and QPM creams were stable at room temperature (28 °C) for 6 months. The skin irritation test in rabbit revealed that both preparations were nonirritant. Furthermore, the in vivo wound healing study in rats showed that CPM and QPM creams had wound healing properties. In conclusion, the curcuminoids and quercetin microcapsules incorporated in creams were successfully prepared. CPM had better wound healing property than QPM.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2573
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCurcuminoids and quercetinen_US
dc.titleComplex Coacervated Microcapsules In Cream For Topical Delivery Of The Curcuminoids And Quercetinen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Farmasi - Tesis