Voltage Clamp Electrophysiology Screening Of Selected Compounds From Zizyphus Mauritiana And Myristica Fragrans On Specific GABA Receptors
| dc.contributor.author | Che Has, Ahmad Tarmizi | |
| dc.date.accessioned | 2019-06-17T06:48:27Z | |
| dc.date.available | 2019-06-17T06:48:27Z | |
| dc.date.issued | 2012-05 | |
| dc.description.abstract | This study focused on the effects of the methanol crude extract of Zizyphus mauritiana leaves and the essential oil of the mesocarp of Myristica fragrans on the function of GABA (A) receptors. Both crude and essential oil were tested on the Xenopus laevis oocytes that comprised of GABA (A) receptors α1β2γ2s subtype. An automated fast perfusion system that applied the two microelectrodes voltage clamp measurement technique at EC5-10 of GABA was used. GABA or gamma-aminobutyric acid is the major inhibitory neurotransmitter in the mammalian central nervous system and involves in the balance between excitation and inhibition in the neuronal cells. GABA expresses the inhibition actions by activation of the GABA receptors. These receptors can be divided into GABA (A), GABA (B) and GABA (C) receptors. GABA (A) receptors are pentameric protein inhibitory receptors that are arranged in a circle to form an intrinsic anion channel permeable to Cl- ions in the middle. These ion channels remain close until opened by specific agonist, GABA and modulated by a variety of compounds that act at its binding sites. The development of new ligands that which has the ability to bind on the GABA (A) receptors binding sites are the primary targets in current therapeutic study. There are many natural substances that have been identified to show potential in GABA (A) receptors activation thus influencing the functions of these ionotropic GABA (A) receptors. When tested at 100 μL/mL the essential oil enhanced I GABA by about 100%, however, the crude of Zizyphus mauritiana leaves at 100 μg/mL showed no statistical significance effect of I GABA. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/8299 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | The methanol crude extract of Zizyphus mauritiana leaves | en_US |
| dc.subject | mesocarp of Myristica fragrans on the function of GABA | en_US |
| dc.title | Voltage Clamp Electrophysiology Screening Of Selected Compounds From Zizyphus Mauritiana And Myristica Fragrans On Specific GABA Receptors | en_US |
| dc.type | Thesis | en_US |
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