The Effect Of Aflatoxin B1 And Ochratoxin A On Tumor Related Genes In Immortalized And Breast Cancer Cells
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Date
2019-02
Authors
Ahmed Adam, Mowaffaq Adam
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Mycotoxin contamination of food commodities caused by fungal strains such
as Aspergillus and Penicillium is common in countries with tropical weather such as
Malaysia due to the conditions that sustains their growth and development.
Mycotoxins have been reported to cause severe food poisoning, liver damage and
were proven carcinogenic to kidney and liver cells. The carcinogenic nature of
Aflatoxin B1 and Ochratoxin A could increase the risks of breast cancer and an
investigation on a molecular level need to be carried out to confirms it. The
objectives of this study are to investigate the effect of AB1 and OTA on cell
activities cytotoxicity, tumor related genes expression and the proliferation of breast
cancer cells at a low and a high concentration. Stock solutions of 100 ug/mL of
Aflatoxin B1 and Ochratoxin A were prepared using DMSO as a solvent and AB1 in
final concentration of 1.2 μg/mL and OTA in final concentration 3.4 μg/mL were
used to treat MCF7. In addition to that, AB1 in final concentration of 2.3 μg/mL and
OTA in final concentration 5.7 μg/mL were used to treat MCF10A cell. Cells were
seeded in the density of 0.3 x 106 and once 90% confluent, cells were treated and
incubated for 48 hours to perform XTT (2H-Tetrazolium, 2,3-bis(2-methoxy-4-nitro-
5-sulfophenyl)-5-[(phenylamino)carbonyl]-hydroxide) cytotoxicity assay, cell
morphology and cell migration assay, cell cycle assay, apoptosis assay and reactive
oxygen species assay. In addition to that, gene annotation and quantification of gene
expression using RT-qPCR was carried out to investigate the expression of tumor
related genes in both cell lines post treatment.
Description
Keywords
The effect of aflatoxin B1 , on tumor related genes