The Effect Of Aflatoxin B1 And Ochratoxin A On Tumor Related Genes In Immortalized And Breast Cancer Cells

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Date
2019-02
Authors
Ahmed Adam, Mowaffaq Adam
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Publisher
Universiti Sains Malaysia
Abstract
Mycotoxin contamination of food commodities caused by fungal strains such as Aspergillus and Penicillium is common in countries with tropical weather such as Malaysia due to the conditions that sustains their growth and development. Mycotoxins have been reported to cause severe food poisoning, liver damage and were proven carcinogenic to kidney and liver cells. The carcinogenic nature of Aflatoxin B1 and Ochratoxin A could increase the risks of breast cancer and an investigation on a molecular level need to be carried out to confirms it. The objectives of this study are to investigate the effect of AB1 and OTA on cell activities cytotoxicity, tumor related genes expression and the proliferation of breast cancer cells at a low and a high concentration. Stock solutions of 100 ug/mL of Aflatoxin B1 and Ochratoxin A were prepared using DMSO as a solvent and AB1 in final concentration of 1.2 μg/mL and OTA in final concentration 3.4 μg/mL were used to treat MCF7. In addition to that, AB1 in final concentration of 2.3 μg/mL and OTA in final concentration 5.7 μg/mL were used to treat MCF10A cell. Cells were seeded in the density of 0.3 x 106 and once 90% confluent, cells were treated and incubated for 48 hours to perform XTT (2H-Tetrazolium, 2,3-bis(2-methoxy-4-nitro- 5-sulfophenyl)-5-[(phenylamino)carbonyl]-hydroxide) cytotoxicity assay, cell morphology and cell migration assay, cell cycle assay, apoptosis assay and reactive oxygen species assay. In addition to that, gene annotation and quantification of gene expression using RT-qPCR was carried out to investigate the expression of tumor related genes in both cell lines post treatment.
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Keywords
The effect of aflatoxin B1 , on tumor related genes
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