Anticancer Studies Of Koetjapic Acid Purified From Sandoricum Koetjape Merr

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dc.contributor.authorNassar, Zeyad D.
dc.date.accessioned2018-08-29T07:45:59Z
dc.date.available2018-08-29T07:45:59Z
dc.date.issued2011-02
dc.description.abstractColorectal cancer which involves the colon, rectum and the anal canal is the third most common malignancy worldwide. The disease is characterized by extensive angiogenesis and perturbation of a variety of oncogenes and tumor suppressor genes including, p53, Wnt, hypoxia, NF-кB, Notch, MAP kinase. Targeting one or more of these pathways increases the selectivity of the chemotherapeutic agents as these pathways are important for cancer cells growth and proliferation. Koetjapic acid is a naturally occurring seco-A-ring oleanene triterpene present in the Sandoricum koetjape species that grows in Malaysia and the Southeast Asian region. In this study, a new method of koetjapic acid purification was developed to produce pure koetjapic acid crystals at high yield. The structure of the compound was resolved and confirmed using various physical and chemical methods which include: X-ray crystallography, 1H NMR, IR, and mass spectra, and melting point determination. The cytotoxic activity of koetjapic acid was assessed using MTT test against four human cancer cell lines namely; colorectal carcinoma (HCT 116), hormone sensitive breast cancer (MCF 7), hormone resistance breast cancer (MDA-MB-231) and liver cancer (Hep G2) cell lines, in addition, two normal human cell line were tested; colon (CCD-18Co) and umbilical vascular endothelial cells (HUVECs) . The results showed that KA is a modest cytotoxic agent but exhibited selectivity against HCT 116 cell line with IC50 18.88 μg/ml. The cellular and molecular mechanisms of anticancer activity of koetjapic acid towards human colorectal cancer were investigated. Here we find that koetjapic acid induces apoptosis in HCT 116 colorectal cancer cells. The early changes accompanying the apoptosis process were investigated by assessment of caspase activation. Koetjapic acid was found to cause activation of downstream and upstream caspases.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6464
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnticancer studies of koetjapic acid purifieden_US
dc.subjectfrom sandoricum koetjape merren_US
dc.titleAnticancer Studies Of Koetjapic Acid Purified From Sandoricum Koetjape Merren_US
dc.typeThesisen_US
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