Novel Methods For The Analytical Determination Of Long Chain Fatty Acids And Vitamin E Homologues In Edible Oil Based Products

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dc.contributor.authorWong, Yong Foo
dc.date.accessioned2018-12-10T02:02:47Z
dc.date.available2018-12-10T02:02:47Z
dc.date.issued2013-09
dc.description.abstractThis thesis deals with the development and validation of new analytical methods for the determination of vitamin E and long chain fatty acids (including the cis/trans isomers of octadec-9-enoic acid). An ultra performance liquid chromatography (UPLC) method for the determination of ten vitamin E components (alpha-, beta-, gamma-, and delta-tocopherol, alpha-, beta-, gamma-, and delta-tocotrienol, alpha-tocopherol acetate, alpha-tocopherol nicotinate) in vegetable oils and margarines is described. The separation was achieved with a KinetexTM pentafluorophenyl column (150 mm × 2.1 mm × 2.6 μm) with both UV and fluorescence (FL) detections operated at 42˚C, under a gradient system consisting of methanol and water at a constant flow rate of 0.38 mL min-1. All the ten compounds were well separated in less than 9.5 min. The method was simple and sensitive with detection limits (signal:noise, 3) of 1.0 to 3.0 μg L-1 (fluorescence detection) and 30 to 74 μg L-1 (UV detection). The method was successfully applied to the determination of vitamin E in vegetable oils and margarines. Capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) was also developed for the simultaneous separation of eleven underivatized fatty acids (FAs), namely, lauric, myristic, tridecanoic (internal standard), pentadecanoic, palmitic, stearic, oleic, elaidic, linoleic, linolenic and arachidic acids. The separation was carried out in normal polarity mode at 20˚C, 30 kV and using hydrodynamic injection (50 mbar for 1s).en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7235
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectNovel Methods For The Analytical Determinationen_US
dc.subjectLong Chain Fatty Acids And Vitamin E Homologuesen_US
dc.titleNovel Methods For The Analytical Determination Of Long Chain Fatty Acids And Vitamin E Homologues In Edible Oil Based Productsen_US
dc.typeThesisen_US
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