Production Of Hyaluronic Acid By Streptococcus Zooepidemicus ATCC 39920

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dc.contributor.authorShoparwe, Noor Fazliani
dc.date.accessioned2018-06-12T07:13:29Z
dc.date.available2018-06-12T07:13:29Z
dc.date.issued2010-04
dc.description.abstractIn view of the demand for hyaluronic acid (HA) products as a starting material for the preparation of new biocompatible and biodegradable polymers that have applications in many medical and cosmetics domains, research on microbial fermentation of HA by S. zooepidemicus ATCC 39920 was undertaken in batch and continuous system. Optimization of fermentation conditions using “one-factor-at-a-time” technique was investigated in shake-flask culture in order to determine the suitable ranges and conditions for use in the study. An inoculum size 10% (v/v), pH 7.0, temperature 37oC, and agitation speed 300 rpm gave the highest specific growth rate, cell biomass and HA production at 0.72 h-1 1.96 gl-1 and 0.82 gl-1, respectively. In order to improve the cell and product yield, optimization of the process parameters were further studied in shake-flask culture using response surface methodology (RSM) coupled with the central composite design (CCD). The optimum conditions obtained from this method were as follows: temperature 37oC, pH 6.96 and agitation 300 rpm, with HA yield per glucose consumed (YHA/glucose) 0.11 gHA gglucose-1 and biomass yield per glucose consume (YX/glucose) 0.19 gcellbiomass gglucose-1, respectively. Temperature and pH have positive effect on accumulation of hydrogen peroxide (H2O2) but insensitive against agitation. H2O2 supported growth and HA production at limited levels (< 10 mM) regardless of H2O2 being released by the cells itself.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5739
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectProduction of hyaluronic acid byen_US
dc.subjectStreptococcus Zooepidemicus ATCC 39920en_US
dc.titleProduction Of Hyaluronic Acid By Streptococcus Zooepidemicus ATCC 39920en_US
dc.typeThesisen_US
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