Whole Genome Sequencing And Characterization Of Three Novel Mangrovimonas-Like Strains Isolated From Mangrove Forests Sediment In Perak, Malaysia
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Date
2017-04
Authors
Balachandra, Dinesh
Journal Title
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Publisher
Universiti Sains Malaysia
Abstract
Strains TPBH4 (=LMG 28913,=JCM 30882), ST2L12 (=LMG 28914,=JCM 30880) and ST2L15 (=LMG 28915,=JCM 30881) were isolated from estuarine sediments in Perak, Malaysia. TPBH4 and ST2L12 were isolated from the estuarine mangrove sediment of Matang Mangrove Forest whereas ST2L15 was isolated from the estuarine mangrove sediment of Taman Paya Bakau. The 16S rRNA gene sequence similarity between TPBH4, ST2L12, ST2L15 and M. yunxiaonensis was 94.9%, 95.3% and 95.2%, respectively. To date, the genus Mangrovimonas is made up of only one type species which has been characterized and identified as M. yunxiaonensis. This strain was isolated from mangrove sediment in China and has had its draft genome published. In this study, the whole genome sequence of these three strains which were closely related to the genus Mangrovimonas were analyzed. The genomes sequenced (3.56 to 4.15 Mb) were relatively larger than that of the type species, M. yunxiaonensis (2.67 Mb). Carbohydrate degradation genes of xylan, xylose, L-arabinan and L-arabinose were uncovered in the genomes of the three Mangrovimonas-like strains. In comparison, these genes were not present in the genome of M. yunxiaonensis. Moreover, strains TPBH4 and ST2L12 has the ability to breakdown xylan which was exhibited through a qualitative plate assay method. Furthermore, these three strains were identified and characterized. TPBH4, ST2L12 and ST2L15 were Gram-negative, yellow/orange/red pigmented, gliding bacteria. Growth pH, salinity and temperature of these three strains were analysed. Furthermore, the respiratory quinone and fatty acids present in these strains were identified. Out of these findings, it was concluded that strain ST2L12 represents a novel species of the genus Mangrovimonas in the family Flavobacteriaceae, with the name Mangrovimonas xylaniphaga sp. nov.
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Keywords
The whole genome sequence , related to the genus Mangrovimonas