Production Of Natural Pigment With Antimicrobial Activity From A Marine Bacterium, Pseudoalteromonas Rubra BF1A IBRL
| dc.contributor.author | Mohd Sulaiman, Azlinah | |
| dc.date.accessioned | 2017-02-10T02:15:07Z | |
| dc.date.available | 2017-02-10T02:15:07Z | |
| dc.date.issued | 2016-02 | |
| dc.description.abstract | Natural pigments are important substitutes to the synthetic dyes in many industrial applications including textile, cosmetics, food, pharmaceutical and also aquaculture industry. Marine bacteria is one of the prolific biological sources for the natural pigments. Besides colouring benefits, many natural pigments posses antimicrobial properties. The search of antimicrobial agents is a continuing dialogue to fight different types of diseases. Thus, the finding of colorant antimicrobial agent can expand the current portrait of the research and can give benefits to many industries since the colorants may give both tinctorial and preservative value simultaneously to the industrial products. The aim of the study is to produce natural pigment by cultivating the marine bacteria in shake flask system, and evaluate the antimicrobial activity of the natural pigment. In this study, a total of 30 cultivable pigmented bacteria were isolated from marine samples, including 8 from macroalgae, 8 from corals, 6 from animal resources, 6 from sand sediment, 1 from seawater and 1 from fish net. Most of the isolated strains were yellow pigmented bacteria (53%), followed by orange (30%), pink (10%) and red pigmented bacteria (7%). Gravimetric analysis of pigment production revealed that all the isolated strains were able to produce different types of coloration with different quantities, where the crude pigment yield ranged from 0.294 g/L to 1.542 g/L. Out of the 30 isolated bacteria, 9 bacteria exhibited antibacterial property against at least one test bacteria. Out of the 9 selected strains, 1 isolate, namely BF1A IBRL was selected as potential strain since it was able to inhibit broader range of test bacteria with lower MIC value, which ranged from 0.28 to 8.88 mg/mL. The extracellular pigment of isolate BF1A IBRL showed higher pigmentation strength with greater antimicrobial activity compared to intracellular pigment. Isolate BF1A IBRL was identified as Pseudoalteromonas rubra based on its phenotypic and genotypic characteristics. The results obtained from UV/vis spectrophotometer, presumptive test and chromatographic analysis indicated that the pigment produced by P. rubra BF1A IBRL is prodigiosin-type. The pigment was red and showed a maximum absorption at 534 nm. Based on the time kill assay the antibacterial activity of prodigiosin extract from P. rubra BF1A IBRL was concentration dependant. The results of bio-guided purification analysis revealed that the purified prodigiosin pigment exhibited greater antimicrobial activity compared to the crude extract. The active fraction (Fraction 4) obtained from column chromatography had similar MIC values with TLC-purified prodigiosin which was 13.75 μg/mL against B. subtilis, B. cereus, MRSA, S. aureus, and A. anitratus. The purified prodigiosin was highly toxic towards the Artemia salina for both acute and chronic toxicities; however the crude extract of the prodigiosin did not cause any toxic effect towards the brine shrimp. The production and evaluation of microbial pigment as textile colorants was investigated. The wool fabrics showed greater affinity towards the prodigiosin extract compared to cotton, silk satin and silk. The naturally dyed wool fabric showed fair to excellent fastness property towards all colourfastness properties except for light colourfastness. Furthermore, the results also disclose that the wool dyed with natural prodigiosin showed 97.21% to 99.99% of bacterial reduction when the fabric was treated with B. subtilis, B. cereus, MRSA, S. aureus A. anitratus and S. epidermidis, whereas 2.83% to 49.8% of bacterial reduction was achieved for E. coli and K. pneumoniae. Overall, the pigment component isolated from Pseudoalteromonas rubra BF1A IBRL has potential as colorant and preservative agent. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3701 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Natural pigments | en_US |
| dc.title | Production Of Natural Pigment With Antimicrobial Activity From A Marine Bacterium, Pseudoalteromonas Rubra BF1A IBRL | en_US |
| dc.type | Thesis | en_US |
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