Immunoregulatory effects of recombinant BCG (rBCG) expressing c-terminus of merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum on mouse macrophage
Loading...
Date
2013-07
Authors
Mohamad, Dhaniah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Macrophage phagocytosis acts as the first line of defence against malaria parasites infection in the innate immune system. Once activated, macrophages act as inflammatory and microbicidal cells through the production of pro-inflammatory cytokines and nitric oxide (NO). In this study, the immunoregulatory effects of a recombinant BCG (rBCG) clone expressing the 19-kDa C-terminus of the merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum was examined on the mouse macrophage cell line J774A.1. The ability of the rBCG clone to stimulate phagocytosis, macrophage viability, expression of toll-like receptors (TLRs) and production of pro-inflammatory cytokines, NO and inducible nitric oxide synthase (iNOS) by the macrophages in the presence or absence of lipopolysaccharide (LPS) or LPS + interferon (IFN)-γ was determined. The results demonstrated that the rBCG clone was able to enhance the phagocytic activity, TLRs expression and production of pro-inflammatory cytokines such as tumor necrosis factor (TNF)- and interleukin (IL)-1 as well as NO and iNOS of the infected macrophages when compared to parent BCG. The results also demonstrated that the rBCG clone reduced the IL-12 p40 production and the viability of the infected macrophages as well as the growth of the rBCG clones when compared to BCG. These immunoregulatory effects are important in innate immune response against malaria parasite. Thus, this rBCG clone may potentially be used to control malaria infection.
Description
Keywords
Macrophage phagocytosis