The Effects Of Eurycoma Longifolia Extract And Eurycomanone On The Steroidogenesis In Mouse Leydig Cells

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dc.contributor.authorKhurshid Ahmed, Nor Amira
dc.date.accessioned2019-03-05T01:49:05Z
dc.date.available2019-03-05T01:49:05Z
dc.date.issued2018-07
dc.description.abstractEurycoma longifolia Jack (Tongkat Ali) is one of the most popular traditional plants found in the tropical rainforest of Southeast Asia, especially in Malaysia and Indonesia. Although E. longifolia is widely used as traditional medicine, little is understood of the in vitro effect on Leydig cells; the testosterone-producing cells. Therefore, the effect of a standardised E. longifolia extract (F2) and pure compound, eurycomanone (EN) on the productions of testosterone, estrogen and aromatase in mouse Leydig cells were determined in this study, whereby the Formestane (FM) was used as a positive control. The effect of F2, EN and FM on the cell viability was first determined using MTT assay. The cell cycle of the treated Leydig cells was then subjected to Propidium iodide (PI) staining prior analysed by Flow Cytometry. The levels of testosterone, estrogen and aromatase in treated Leydig cells were then determined by ELISA. Finally, the aromatase enzyme activity was screened using a high throughput inhibitor screening kit. The study found that the growth inhibitory effect increased in a dose-dependent manner in the Leydig cells treated with F2. However, this phenomenon was not observed in EN- and FM-treated cells despite when higher concentrations were used. Cell cycle analysis revealed that F2-treated cells reduced viability due to the cell cycle arrest at S phase within 96 hours treatment. A significant S phase cell cycle arrest at 24 hours and 48 hours treatments could also be observed in EN-treated cells. However, the arrested cells were reduced at 72 hours and 96 hours treatments. Meanwhile, FM-treated cells did not show any significant cell cycle arrest. The ELISA revealed that F2-, EN- and FM-treated Leydig cells increased the level of testosterone may be due to the reduction of the conversion of testosterone to estrogen.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7819
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThe effects of eurycoma longifolia extract and eurycomanoneen_US
dc.subjecton the steroidogenesis in mouse leydig cellsen_US
dc.titleThe Effects Of Eurycoma Longifolia Extract And Eurycomanone On The Steroidogenesis In Mouse Leydig Cellsen_US
dc.typeThesisen_US
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