Changes In Zebrafish (Danio Rerio) Liver Protein Expression Due To Starvation. A Proteomics Approach For Analysis Of Crude Mitochondrial Extract.

Loading...
Thumbnail Image
Date
2011-05
Authors
Enyu, Yee Ling
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Understanding the metabolic mechanism changes of organisms during starvation has raised the interest of scientists over the past years. The association of mitochondrion with related functions such as energy production, fatty acids metabolism, life span extension and ageing process has made it a target organ in this study. With this in mind, this study was designed to look into the changes of the crude mitochondrial extract after a period of starvation in adult zebrafish liver. Second dimension sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) and gel analysis revealed 99 spots undergoing changes in expression pattern during 15 days of food deprivation. A total of 35 spots which fulfilled three important criteria were selected for mass spectrometry; A) can be detect in all gels, B) shows a consistent expression pattern, and C) intensity of spots must be significantly different from the comparison sample in all replicates. Nineteen spots were positively identified from 35 spots sent for analysis. Interestingly, results showed that several proteins involved in chaperoning and anti-oxidative reactions such as heat shock proteins, peroxiredoxin and paraoxonase were upregulated while a protein involved in the catabolic pathway was downregulated during starvation. These responses lead to lower metabolic activities, slowdown of ageing process and extension of life-span. Several proteins involved in fatty acid and amino acid metabolism were upregulated indicating a majority of energy supplies during prolong starvation were not from a carbohydrate source. As expected, results showed an overall reduction in glycolysis and an increase in gluconeogenesis during starvation.
Description
Keywords
Zebrafish (Danio Rerio) Liver , Crude Mitochondrial Extract.
Citation