Changes In Zebrafish (Danio Rerio) Liver Protein Expression Due To Starvation. A Proteomics Approach For Analysis Of Crude Mitochondrial Extract.
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Date
2011-05
Authors
Enyu, Yee Ling
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Understanding the metabolic mechanism changes of organisms during
starvation has raised the interest of scientists over the past years. The association of
mitochondrion with related functions such as energy production, fatty acids
metabolism, life span extension and ageing process has made it a target organ in this
study. With this in mind, this study was designed to look into the changes of the
crude mitochondrial extract after a period of starvation in adult zebrafish liver.
Second dimension sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D
SDS-PAGE) and gel analysis revealed 99 spots undergoing changes in expression
pattern during 15 days of food deprivation. A total of 35 spots which fulfilled three
important criteria were selected for mass spectrometry; A) can be detect in all gels, B)
shows a consistent expression pattern, and C) intensity of spots must be significantly
different from the comparison sample in all replicates. Nineteen spots were
positively identified from 35 spots sent for analysis. Interestingly, results showed that
several proteins involved in chaperoning and anti-oxidative reactions such as heat
shock proteins, peroxiredoxin and paraoxonase were upregulated while a protein
involved in the catabolic pathway was downregulated during starvation. These
responses lead to lower metabolic activities, slowdown of ageing process and
extension of life-span. Several proteins involved in fatty acid and amino acid
metabolism were upregulated indicating a majority of energy supplies during prolong
starvation were not from a carbohydrate source. As expected, results showed an
overall reduction in glycolysis and an increase in gluconeogenesis during starvation.
Description
Keywords
Zebrafish (Danio Rerio) Liver , Crude Mitochondrial Extract.