Callus And Cell Culture Of Gynura procumbens (Lour.) Merr. (compositae) Using Stationary Culture System
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Date
2015-06
Authors
Khaw, Mei Lin
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Abstract
The potential use of fabric as immobilization units for the callus and cell culture of Gynura procumbens was evaluated. Four different types of fabrics (linen, muslin and cotton 1 and cotton 2) were chosen for the study and their pore size was also examined. Linen was found to be the best support agent for the callus culture of G. procumbens when cultured on square pieces of fabric cut into 5 cm x 5 cm. The fresh biomass yield was 3.022 ± 0.108 g. The callus produced from culturing on fabric was yellowish, friable and granular in nature. The pore size of linen generally covers a wider range from 0.012 mm2 – 0.071 mm2 compared to other three types of fabrics when examined under stereomicroscope. When the callus of G. procumbens was cultured using linen of different design (bag or piece) and different medium volume (10 mL, 15 mL, 20 mL, 25 mL and 30 mL) under stationary mode, the highest callus biomass yield (2.733 ± 0.153 g) was obtained from callus cultured using linen pieces in 10 mL liquid medium. The callus was yellowish in color and their texture was friable with granule. A lesser amount of liquid culture medium generally supported higher callus fresh biomass gain of G. procumbens coupled with a healthy callus growth. The callus cultured under continuous light with light intensity of 2000 – 2500 lux was more friable and less compact. The highest biomass yield (1.381 ± 0.458 g) and healthy friable callus were obtained from callus cultured in 10 mL culture medium under stationary mode using muslin bag (5 cm x 5 cm). The vessel size was found to have significant effect on the cell growth of G.procumbens. The cells cultured in 500 mL vessel for 12 days produced the highest and healthy fresh cell biomass (5.161 ± 0.747 g/L) of G. procumbens. Hence, this study indicated that the callus of G. procumbens could be maintained on pieces of linen in 10mL of MS medium under stationary mode. It also showed that 500 mL vessel is an ideal size for the optimal cell growth of G. procumbens.
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Natural History