Modification Of A Cell Culture Tank For Higher Production Of Cell Biomass Using Cyperus aromaticus As A Model
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Date
2015-07
Authors
Omar, Mohamed Taufiq
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A modification study was carried out on the 10 L fabricated plant cell culture tank to make it more versatile and useful for cell cultures of other plant species. Hence, this study was carried out to modify the existing cell culture tank systems for enhanced production of cell biomass by using Cyperus aromaticus as a model. Other than focusing on improving productivity of the tested cells, the present study also highlighted and detailed the basic modification aspects in developing a well designed culture tank system to achieve contamination-free operation. An effective sequential sterilization procedure of the cell culture tank, a combination of chemical (95 % ethanol) soaking followed by dry heat sterilization at 150 °C was successfully developed. The sterile tank establishment has been supported by several comprehensive modification strategies. The previous unsatisfactory inoculation system was replaced with closed and sterile systems; peristalsis mechanism and pouring method which respectively used for large cell aggregates and small aggregates or fine cells. Besides that, the sterile boundary integrity of the vessel was preserved with the installation of new mechanical seal (Teflon bushing and rust-proof ball bearing) of the impeller shaft. In addition, the challenge of maintaining aseptic condition during continuous aeration and periodical sampling was overcome with the segregation and modification of aeration and sampling system. Effective inlet air sterilization was successfully attained via dual filtration system which
embodied the application of UV column sterilization and ultra filtration. Biomass productivity of C. aromaticus cells was enhanced with the application of the modified cell culture tank together with the improved physical culture conditions. The highest biomass productivity was achieved when C. aromaticus cells were moderately mixed at 80 rpm using marine blade impeller, continuously aerated at 0.15 vvm via stainless steel sintered sparger with addition of 2.5 ml/5 L antifoam A concentrate in the culture medium for foam mitigation and maintained at temperature of 35 ± 2 °C. Nonetheless, these improved physical conditions were unfavourable to the cells of selected plant species, Orthosiphon stamineus, Eurycoma longifolia and Artemisia annua as unsatisfactory cell growth was exhibited. Hence, further optimisation studies on individual plant species are essential.
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Keywords
Modification Of A Cell Culture Tank , For Higher Production Of Cell Biomass.