Identification Of Helicobacter Pylori Antigenic Proteins From Isolates Of Patients With Gastric Pathologies
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Date
2011-07
Authors
Lee, Chun Wei
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Helicobacter pylori is a gram negative, spiral-shaped, microaerophilic bacterium, which
colonizes the apical side of human gastric epithelial cells and mucous layer. H. pylori
infections are associated with various gastric diseases with wide spectrum of clinical
outcomes, ranging from gastric cancer to ulceration of the gastrointestinal tract. The
pathogenicity of the infection depends on the strain virulence, host susceptibility and
environmental co-factors. Thus it is very important to understand the pathogenesis of the
disease. Identification of the antigenic proteins of H. pylori, may eventually lead to the
development of diagnostic test for this pathogen.
Several media were evaluated for isolation and propagation of H. pylori. Three
clinical isolates were used and ATCC 700824 strain were employed as reference. For
liquid medium, Brain Heart Infusion broth and Tryptic Soy broth with 10% fetal bovine
serum were tested and the former was found to give higher microbial growth (cfu).
Meanwhile comparisons between serum or blood supplemented solid media i.e. Tryptic
Soy Agar (TSA), Columbia Agar, Eugon Agar and Brain Heart Infusion Agar (BHIA),
showed that the media with blood supplement gave higher cfu. The conventional agar
medium, TSA with 5% defibrinated sheep blood, produced the best growth among the
solid media.
In this study, stomach biopsy samples were from the patients who underwent
Esophageal-Gastro-Duodenal scope. Out of 564 biopsy samples, 50 isolates of H. pylori
were successfully isolated from patients with various gastric pathologies. PCR screening
showed that all isolates were positive for Ure C gene, whereas only 42 isolates were
positive for Cag A gene.
Description
Keywords
Identification of helicobacter pylori Antigenic proteins , Isolates of patients with Gastric pathologies