Identification Of Helicobacter Pylori Antigenic Proteins From Isolates Of Patients With Gastric Pathologies

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Date
2011-07
Authors
Lee, Chun Wei
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Publisher
Universiti Sains Malaysia
Abstract
Helicobacter pylori is a gram negative, spiral-shaped, microaerophilic bacterium, which colonizes the apical side of human gastric epithelial cells and mucous layer. H. pylori infections are associated with various gastric diseases with wide spectrum of clinical outcomes, ranging from gastric cancer to ulceration of the gastrointestinal tract. The pathogenicity of the infection depends on the strain virulence, host susceptibility and environmental co-factors. Thus it is very important to understand the pathogenesis of the disease. Identification of the antigenic proteins of H. pylori, may eventually lead to the development of diagnostic test for this pathogen. Several media were evaluated for isolation and propagation of H. pylori. Three clinical isolates were used and ATCC 700824 strain were employed as reference. For liquid medium, Brain Heart Infusion broth and Tryptic Soy broth with 10% fetal bovine serum were tested and the former was found to give higher microbial growth (cfu). Meanwhile comparisons between serum or blood supplemented solid media i.e. Tryptic Soy Agar (TSA), Columbia Agar, Eugon Agar and Brain Heart Infusion Agar (BHIA), showed that the media with blood supplement gave higher cfu. The conventional agar medium, TSA with 5% defibrinated sheep blood, produced the best growth among the solid media. In this study, stomach biopsy samples were from the patients who underwent Esophageal-Gastro-Duodenal scope. Out of 564 biopsy samples, 50 isolates of H. pylori were successfully isolated from patients with various gastric pathologies. PCR screening showed that all isolates were positive for Ure C gene, whereas only 42 isolates were positive for Cag A gene.
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Keywords
Identification of helicobacter pylori Antigenic proteins , Isolates of patients with Gastric pathologies
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