Ecology, Diversity, Antibiotic Resistance Of Lactic Acid Bacteria (LAB) Isolated From Plant Sources And Their Role In Natural Fermentation Of Tempoyak

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dc.contributor.authorChuah, Li Oon
dc.date.accessioned2019-08-20T01:50:08Z
dc.date.available2019-08-20T01:50:08Z
dc.date.issued2016-11
dc.description.abstractIn recent years, bioprospecting of lactic acid bacteria (LAB) from plant sources contributes to the diversity of LAB and the discovery of novel application of LAB in food industries. As LAB play an important role in food fermentation, there are concern of dissemination of antibiotic resistance determinants harboured by the indigenous microbiota present in fresh fruits and fermented food through the food chain. The objectives of this study were to investigate the diversity and antibiotic resistance of LAB presence in fresh tropical fruits and flowers, as well as tempoyak produced by natural fermentation; to characterise the bacteriocin-like substances (BLIS) produced by these LAB isolates, and other technological properties. This study was also undertaken to determine the dynamics of different LAB species during natural fermentation of tempoyak. Based on phylogenetic analysis, phenotypic characterisation and comparison of average nucleotide identity (ANI), a novel genus with two species belonging to the family Streptococcaceae, were identified. The name of the new genus and two species were proposed as Anthococcus rusulii (isolated from durian flowers and hibiscus) and Anthococcus penangensis (isolated from hibiscus flowers). Members of the genera Lactobacillus, Weissella, Lactococcus, Leuconostoc, and Enterococcus were also isolated from fresh tropical fruits and flowers. Genotyping using PFGE demonstrated a high polymorphism among the predominant Lactobacillus plantarum and Weissella spp. isolated from flowers and fruits. The present study also highlighted the concern of dissemination of antibiotic determinants harboured by indigenous microbiota present in fresh fruits through the food chain. Multidrug- resistant LAB isolates harbouring determinants responsible for multiple antibiotics resistance were isolated from fresh fruits.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8632
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAntibiotic resistance of Lactic Acid Bacteriaen_US
dc.subjectnatural fermentation of tempoyaken_US
dc.titleEcology, Diversity, Antibiotic Resistance Of Lactic Acid Bacteria (LAB) Isolated From Plant Sources And Their Role In Natural Fermentation Of Tempoyaken_US
dc.typeThesisen_US
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