Behavioural Pharmacology Of Mitragyna Speciosa Korth Standardized Methanol Extract In An Animal Model
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Date
2011-11
Authors
Senik @ Nawi, Mohd Harizal
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Publisher
Universiti Sains Malaysia
Abstract
Mitragyna speciosa Korth is one of the species of plant used by locals especially in Thailand and Malaysia in treating diarrhea, worm infestations and acts as an analgesic and antipyretic. There is scarcity of information in the literature regarding to toxicity, pharmacology and behaviour effect of this indigenous medicinal herb on. Therefore, this study was conducted to determine the acute toxicity level of Mitragyna speciosa Korth methanol extract (MS) in vivo, to evaluate cognitive functions of rats after acute exposure of MS using avoidance tasks and to delineate the effect of MS in inducing neuronal potentiation of rats’ hippocampus cells using extracellular recording. Rats were orally administered single dose of 100, 500 and 1000 mg/kg of MS, the positive control group received 430 mg/kg of morphine and negative control group received 2 mL vehicle (CMC) orally. Eight parameters were tested; cage side observation, body weight measurement, food and water consumption, blood pressure, absolute and relative organ weight, haematology, biochemical analysis and histopathology. Learning and memory were evaluated using one-way passive avoidance test and two-way active avoidance test. Extracellular recording of field excitatory postsynaptic potential were run to get IC50 from various concentration of MS (0.0001%, 0.001%, 0.005%, 0.01%, 0.05% and 0.1%). Long-term potentiation responses were evaluated using the concentration of IC50. No mortality was observed after 14 days of treatment; only 2 rats from morphine group died. No significant changes were detected in behaviour, food and water consumption, haematological values and organ weights. Rats from the vehicle group showed normal blood pressure however, significant decreases (p < 0.05) of blood pressure were detected in rats from morphine group.
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Keywords
Mitragyna speciosa Korth is species of plant , used by locals especially in treating diarrhea