The Effect Of Gold Nanoparticle Size In Breast Cancer MCF-7 Cell Line Treatment By X-Ray And Linear Accelerator (LINAC)
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Date
2017-05
Authors
Rosli, Nur Shafawati
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Gold Nanoparticles (AuNPs) has been used to enhance the radiation effects in
MCF-7 breast ardenocarcinoma cell line by radiotherapy equipments. AuNPs can be
used during radiation therapy to kill cancer cells in order to reduce the dose of
radiation to kill the tumours, while at the same time sparing the normal tissue. The
irradiation were done using superficial kilovoltage X-rays and megavoltage photon
beams in radiotherapy which are via X-ray machine and Linear Accelerator
(LINAC). In this work, MCF-7 cells are seeded in the 96-well plate and were treated
with three different sizes of AuNPs (13 nm, 50 nm, and 70 nm) before they were
irradiated with 80 kVp and 20 mAs X-ray beams, 6 MV, and 10 MV photon beam at
various radiation doses. To validate the enhanced killing effect, both with and
without AuNPs MCF-7 cells is irradiated simultaneously. Then, the irradiated MCF-
7 cells were incubated at 37 °C and 5% CO2 in a humidified atmosphere incubator
for 24 hours. The absorbance of the irradiated cells were detected with WST-1 cell
proliferation assay 24 hours after irradiation and then, the cell survival fractions were
calculated. The results show that AuNPs significantly enhance cancer killing by
reducing the survival fraction of the cell. Means that, AuNPs enhance the radiation
effects to the cells in either irradiation with kilovoltage superficial X-rays, or
megavoltage photon beams radiotherapy. Additionally, the obtained cell survival
curves also lend a strong support to the argument that the size of AuNPs also plays
important role in determining the enhancement radiation effects.
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Keywords
The effect of gold nanoparticle size by , breast cancer MCF-7 cell line treatment