The Effect Of Gold Nanoparticle Size In Breast Cancer MCF-7 Cell Line Treatment By X-Ray And Linear Accelerator (LINAC)

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dc.contributor.authorRosli, Nur Shafawati
dc.date.accessioned2019-08-29T06:55:25Z
dc.date.available2019-08-29T06:55:25Z
dc.date.issued2017-05
dc.description.abstractGold Nanoparticles (AuNPs) has been used to enhance the radiation effects in MCF-7 breast ardenocarcinoma cell line by radiotherapy equipments. AuNPs can be used during radiation therapy to kill cancer cells in order to reduce the dose of radiation to kill the tumours, while at the same time sparing the normal tissue. The irradiation were done using superficial kilovoltage X-rays and megavoltage photon beams in radiotherapy which are via X-ray machine and Linear Accelerator (LINAC). In this work, MCF-7 cells are seeded in the 96-well plate and were treated with three different sizes of AuNPs (13 nm, 50 nm, and 70 nm) before they were irradiated with 80 kVp and 20 mAs X-ray beams, 6 MV, and 10 MV photon beam at various radiation doses. To validate the enhanced killing effect, both with and without AuNPs MCF-7 cells is irradiated simultaneously. Then, the irradiated MCF- 7 cells were incubated at 37 °C and 5% CO2 in a humidified atmosphere incubator for 24 hours. The absorbance of the irradiated cells were detected with WST-1 cell proliferation assay 24 hours after irradiation and then, the cell survival fractions were calculated. The results show that AuNPs significantly enhance cancer killing by reducing the survival fraction of the cell. Means that, AuNPs enhance the radiation effects to the cells in either irradiation with kilovoltage superficial X-rays, or megavoltage photon beams radiotherapy. Additionally, the obtained cell survival curves also lend a strong support to the argument that the size of AuNPs also plays important role in determining the enhancement radiation effects.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8730
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThe effect of gold nanoparticle size byen_US
dc.subjectbreast cancer MCF-7 cell line treatmenten_US
dc.titleThe Effect Of Gold Nanoparticle Size In Breast Cancer MCF-7 Cell Line Treatment By X-Ray And Linear Accelerator (LINAC)en_US
dc.typeThesisen_US
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