Analysis Of Haruan (Channa Striatus) Water Extract And The Study Of Haruan Aerosol On Wound Healing

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dc.contributor.author., Lia Laila
dc.date.accessioned2018-12-28T02:47:01Z
dc.date.available2018-12-28T02:47:01Z
dc.date.issued2011-11
dc.description.abstractThe enhancement of wound healing properties of haruan water extract has been studied. The in vitro and in vivo studies using cell line and animal model were carried out to evaluate the effectiveness of the extract. Analytical study of haruan water extract was conducted to determine the amino acid and fatty acid contents. The determination of the amino acid in haruan water extract was carried out using acid hydrolysis method, followed by AQC pre-column derivatization and analysed using high performance liquid chromatography equipped with fluorescence detector, while the fatty acid determination was performed using acid methylation method with gas chromatography equipped with flame ionization detector. A method validation was also established to ensure the data obtained reliable and valid for both amino acid and fatty acid analysis. The results of validation parameters showed that all the data obtained were in the range of acceptable value accordance to ICH guideline. The results showed that haruan water extract containing at least 16 amino acids of which glycine and glutamic acid were the major component of the amino acids (17.51% and 14.32% respectively of total amino acid) in the extract. While, tyrosine followed by histidine was the least amino acids. The fatty acid analysis showed that there were at least 18 fatty acids identified in haruan water extract which were categorized as saturated fatty acid, monounsaturated fatty acid, and polyunsaturated fatty acid. The most abundant fatty acid in haruan water extract is palmitic acid followed by palmitoleic acid (39.12% and 11.28% respectively of total fatty acid). While lauric acid and erucic acid is the least fatty acid found in the extract. The in vitro study was carried out using wound healing scratch assay to evaluate the effect of haruan water extract in the migration of wounded cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7413
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnalysis of haruan water extracten_US
dc.subjectharuan aerosol on wound healingen_US
dc.titleAnalysis Of Haruan (Channa Striatus) Water Extract And The Study Of Haruan Aerosol On Wound Healingen_US
dc.typeThesisen_US
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