Xylanase Production By Phanerochaete Chrysosporium ME 446 (ATCC 34541) In A Submerged Culture System
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Date
2001-12
Authors
Obeidat, Sattam Mohammad Ali
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Abstract
Optimization of cultural conditions and medium compositions for xylanase production by
free and immobilized cells of P. chrysosporium were carried out in a shake flask system.
The optimized cultural conditions. with the cultivation temperature of 30°C, agitation
speed at 150 rpm, inoculum size .. ::.f 6 x 106 spores/ml and initial medium pH of 5.0
yielded about 3.5 IU/ml of xylanase activity and 0.52 gil of fungal biomass after six days
of cultivation. The optimization of =tedium composition was also carried out by selection
of better carbon, nitrogen and inducer sources for enhancing the xylanase production ..
The optimized medium composition. which consisted of 0.1 0% (w/v) cellulose, 0.1%
(w/v) ammonium chloride and 0.1 ~ t (wi\") xylan as carbon, nitrogen and inducer sources,
respectively, produced about 17.8 :-.J/ml of xylanase activity and about 0.76 gil of the
fungal biomass.
In order to enhance the production. of xylanase and to protect the fungal cells from
abrasion and shear forces during egitation in the optimized cultivation medium, the
immobilization technique using scouring mesh cubes and calcium alginate beads as
support materials were performed. The maximum xylanase production produced by the
scouring mesh 'and calcium alginate immobilized cells were about 80.2 IU/ml and 32.4
IU/mI, respectively. There were abc·::! 400% and 180% increment of xylanase production
by scouring mesh and calcium alginate immobilized cells, respectively, compared to free
cells. The scouring mesh irnmobilized cells of P. chrysosporium exhibited a significant of
two repeated run.
The immobilized cells of P. chrysosporium in scouring mesh cubes was then cultivated in
a 2.0 L stirred tank fementer system for 10 days. The results exhibited the xylanase
production of about 159.3 IU/ml after 8 days of cultivation in an optimized medium with
150 pieces of 0.75 cm3 scouring mesh cubes, agitation speed of 150 rpm, aeration rate of
1.0 vvrn and inoculum size of 2.5% (w/v) of 6 x 106 spores/ml.
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Keywords
Optimization of cultural conditions and medium compositions for , xylanase production by free and immobilized cells of P. chrysosporium