Agen antikulat (antikandida) darjpada cinnamomum zeylanjcumblume
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Date
2004
Authors
Nordin, Noor Hazarina
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Abstract
The aim of the study was to investigate the anticandidal activity of various medicinal
plant species. In this study, chloroform extracts from 23 plant species were screened.
The strongest anticandidal activity was shown by the C. zeylanicum extract, with the
lowest MIC and MLC values of 0.391 mg/ml and 0.781 mg/ml, respectively. Hence,
further study was only focused on C. zeylanicum. Observation of the yeast's growth
profile exposed to a certain extract concentration, revealed that the growth of C. albicans
was completely inhibited. This finding was supported by light microscope, scanning
electron microscope and transmission electron microscope micrographs that showed
various changes occurring on the structure and morphology of the extract-treated yeast
'
cells.
The results suggested that the anticandidal agent interfere in the permeability of the cell
membrane and cell wall biosynthesis of the yeast cells, which subsequently lead to the
formation of cytopathological :and membrane structural degeneration and finally leading
to cell lysis and death. Cytotoxicity tests of the extract by brine shrimp lethality assaY.
showed that the significant value of lethality concentration of 50 (LC5o) was 0.2 mglml.
Several components were isolated from the crude extract of C. zeylanicum by column
chromatography methods. From the total 42 samples of isolated components, only one
showed anticandidal activity against C. albicans. The difference in the inhibition zone
sizes formed by the isolated component and the crude extract itself suggested that the
extract showed maximum inhibition when all bioactive compounds act together and
generate sinergisme effects against C. albicans.
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Keywords
Antikandida , Cinnamomum zeylanjcumblume