Touch dna profiling from processed fingerprint
Loading...
Date
2009
Authors
Hafidza, Baharum
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
Abstract
Forensics identification using fingerprint analysis is only possible if there is a complete pattern of ridges obtained from the crime scene. In this study we have attempted to perfonn DNA analysis on processed smudged fingerprints. A subject was requested to deposit complete and portioning fingerprints on glass surface. The deposited fingerprints were exposed to 18 •c, 28 OC, 34 •c, and 40 OC for an hour, 3 days, and 7 days. The fingerprints were then visualized using black powder, were measured and one of sample was recovered using tape lifting method. A total of 2t.11 of the extracted DNA was quantitated using Real Time PCR (Quantifiler kit). 2 samples with DNA concentration above 1.0 ng was used for amplification using Amp Ff.STR ldentifiler kit and another 2 samples concentration above 0.2 ng was used for amplification using AmpFtSTR Minifiler kit. Both kits failed to amplify with DNA and therefore optimization is required for the collection and extraction methods used in this study.
Description
Keywords
Forensics