Analisis variasi genetik dan morfologi beberapa kultivar jambu batu (psidium guajava) di Semenanjung Malaysia
Loading...
Date
2008-11
Authors
Abdul Wahab, Mohammad Sarifudin
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A morphological and molecular genetic study was conducted on guava
cultivars obtained from various parts in Peninsular Malaysia. This is the first
documentation to determine the similarities, differences and relationships
between each guava cultivars utilizing morphological and molecular techniques.
The guava cultivars investigated were Skg (seeded), KbsD (seeded), Batu
(seeded), KbG (seeded), SdB (seeded), Aiss (seedless), Epal (seedless), Sds
(seedless) and Bulat (seedless). A total of 180 samples consisting of 9 guava
cultivars were used for physical morphometric measurement. All of the 6 physical
morphological characters that use are namely MSp (mesocarp), EDp (endocarp),
Brt (weight), Lbr (leaf Lenght2), Pjg 1 (leaf Lenght1 ), and Dmt (diameter). These
physical morphometric measurement are use for supported the genetic result
from Random Amplified polymorphic DNA (RAPD), where all 180 of the same
plant samples were used. RAPD analyses were carried out using 5 different
primers, OPB-04, OPB-18, OPF-03, OPF-09 and OPG-1 0, which produce a total
of 68 polymorphic bands. Neighbor-Joining (NJ) cluster analysis generated a
dendrogram, which successfully divided guava cultivars into nine clusters. More
detailed studies of the physical characteristics and genetic population studies are
suggested to have a better understanding of guava similarities and differences
between cultivars. The differences of guava cultivars shown in molecular
analyses can also be seen in their physical character more clearly.
Description
Keywords
Variasi genetik , Jambu batu (psidium guajava)