Biodiversity And Phylogenetic Studies Of Land Snails (Pulmonata: Stylommatophora) In Peninsular Malaysia
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Date
2013-12
Authors
Abu Bakar, Siti Balkhis
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A total of 24 species from 21 genera in 14 families sampled from 19 locations
in Peninsular Malaysia were identified by looking at their shell morphological
characteristics and compared with voucher specimens in Natural History Museum,
London. All the specimens were successfully sequenced based on the double
amplification of rRNA genes that comprised of the conserved and variable regions
(5.8S gene, the complete ITS-2 region and the large subunit 28S gene). These
Malaysian taxa sequences were then analyzed together with available 179
Stylommatophoran data based on 844 bp sequences to investigate their phylogenetic
relationships. Both Neighbour-Joining (NJ) and Maximum Likelihood (ML)
phylogenetic trees remained monophyletically, supported (as in previous analysis)
with the splitting into ‘achatinoid’ and ‘non-achatinoid’ clades (40% and 100% in NJ
and ML bootstrap values, respectively) with the Malaysian inclusion. The
‘achatinoid’ clade had four distinct and stable clusters’ namely (1) Plectopylidae, (2)
Streptaxidae and Diapheridae (Streptaxoidea), (3) Ferussaciidae, and (4) a lineage
that includes Subulinidae, Achatinidae, Coeliaxidae and Thyrophorellidae. From the
seven major groups in the ‘non-achatinoid’ clade, the taxonomic status of
Helicoidea, Orthalicoidea, Elasmognatha and Orthurethra remained unresolved as no
additional or only a single representative taxon was obtained in the present study.
However, groups within the Limacoidea, Clausilioidea and Arionoidea taxa require
further investigations that may lead to taxonomic revisions.
Description
Keywords
Biodiversity And Phylogenetic , (Pulmonata: Stylommatophora)