Anticancer Effects Of Tualang Honey On 7,12- Dimethylbenz(α)Anthracene (DMBA)-Induced Mammary Carcinoma In Rats

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Date
2011
Authors
Abd Kadir, Erazuliana
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Publisher
Universiti Sains Malaysia
Abstract
Tualang honey (TH) has been reported to have antiproliferative effect and induces apoptosis in various cancer cell lines. The present study was conducted to evaluate the effects of TH on the initiation and progression of 7,12- dimethylbenz(α)anthracene (DMBA)-induced mammary carcinoma in rats. A preliminary study was conducted to establish the optimum DMBA dose for cancer induction. Single administration of 20 or 25 mg DMBA to 45 to 48 days old female rats with bodyweight of less or more than 120 g respectively was able to induce 50% carcinoma incidence. Later, 40 DMBA induced rats were block randomized into four groups; Group 1, 2, 3 and 4 and given 0 (control), 0.2, 1.0 or 2.0 g/kg/day of TH respectively starting the next day for 150 days. Breast tumor nodules were assessed twice weekly and body weight was measured once a week. On Day 151, blood was withdrawn for analysis of total lymphocyte count and later the animals were euthanized. During autopsy, the tumor incidence, criteria, amount, weight and volume were assessed. Tumors were then subjected for histopathological examination and determination of apoptosis index and vascular endothelial growth factor level. Clinical observation revealed that Group 2 presented with the highest percentage of tumor incidence (75%) whereas Group 4 with the lowest (42.9%). Group 4 also had the shortest tumor latency period (Day 101 ± 18) while Group 3 had the longest (Day 140 ± 4). TH-treated groups (THG) showed lower percentage of actual body weight gain than control (p = 0.012). Tumors in THG presented with slower size increment and smaller mean tumor size (≤2 cm3) compared to control (≤8 cm3). Number of tumors during autopsy in THG was lesser (12, 5 and 7 tumors for Group 2, 3 and 4 respectively) than control (14 tumors). Total tumor weight and volume per animal were also lower in THG. Tumor weight in Group 2 and tumor volume in Group 4 were significantly lower than control (p = 0.048 and p = 0.018 respectively).
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Keywords
Tualang honey have antiproliferative effect , induces apoptosis in various cancer cell lines
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