Molecular Analysis And Protein Quantification Of Rh Blood Group System Among Blood Donors At The National Blood Centre, Malaysia
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Date
2014-08
Authors
Musa, Rozi Hanisa
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Publisher
Universiti Sains Malaysia
Abstract
Rh is the most polymorphic and immunogenic human blood group system, with over 50 antigens now identified. The Rh antigens are located on two proteins, RhD and RhCE: the former carries the D antigen, whilst the latter carries the C, c, E and e antigens. These antigens can be further subdivided into various genotypes. The clinical significance of the Rh antigen is related to the ability of D negative or some D variant types to form anti-D if exposed to D antigens. Anti-D is a major cause of Haemolytic Disease of the Fetus and Newborn (HDFN) and also can cause Haemolytic Transfusion (HTR). Multiple factors can complicate the determination of the D status which include different methods and reagents used that can react differently with the polymorphic RHD genes. Thus, applications of molecular techniques for the detection of Rh antigens are useful for reducing the risk of alloimmunization. The aim of this study was to characterize the Rh genes and its corresponding Rh antigens protein quantification among blood donors at the National Blood Centre (NBC), Kuala Lumpur, Malaysia. The study subjects were 1014 blood donors comprising of 360 Malays, 434 Chinese, 164 Indians, and 56 from other minor ethnic groups. Blood samples collected were serologically phenotyped using automated machine, Olympus PK7200. Then these samples were subjected to molecular analysis by applying the PCR- Sequence Specific Primers (PCR-SSP) technique. Protein quantification and automated sequencing were performed on 120 samples with results that showed discrepancies from the serological and molecular analysis.
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Keywords
Molecular analysis and protein quantification , of Rh blood group system