eDNA CLONES CONSTRUCTION OF DENGUE VIRUS TYPE-2 (NEW GUINEA C-STRAIN)

dc.contributor.author	KEAT, ONGENG
dc.date.accessioned	2016-01-12T03:26:46Z
dc.date.available	2016-01-12T03:26:46Z
dc.date.issued	2009-04
dc.description.abstract	Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells infected with DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse transcribed and amplified as seven overlapping fragments, covering the whole genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of these fragments was successfully cloned into a high copy number plasmid, pGEM®T vector and transformed into Escherichia coli (E. coli) JMl 09 strain. The identity of each clone was verified using DNA sequencing. The seven overlapping fragments were successfully joined together in pGEM®-T vector and transformed into E. coli JM109 strain at 25°C with the presence of ampicillin (25 !Jglml) to produce a eDNA clone carrying the full-length genome ofDEN-2.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/1369
dc.subject	eDNA CLONES CONSTRUCTION OF DENGUE VIRUS TYPE-2	en_US
dc.title	eDNA CLONES CONSTRUCTION OF DENGUE VIRUS TYPE-2 (NEW GUINEA C-STRAIN)	en_US
dc.type	Thesis	en_US

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